Long term anoxia in rainbow trout investigated by 2-DE and MS/MS.

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Long term anoxia in rainbow trout investigated by 2-DE and MS/MS. / Wulff, Tune; Jessen, Flemming; Roepstorff, Peter; Hoffmann, Else Kay.

In: Proteomics, 2008, p. 1009 - 1018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wulff, T, Jessen, F, Roepstorff, P & Hoffmann, EK 2008, 'Long term anoxia in rainbow trout investigated by 2-DE and MS/MS.', Proteomics, pp. 1009 - 1018. https://doi.org/10.1002/pmic.200700460

APA

Wulff, T., Jessen, F., Roepstorff, P., & Hoffmann, E. K. (2008). Long term anoxia in rainbow trout investigated by 2-DE and MS/MS. Proteomics, 1009 - 1018. https://doi.org/10.1002/pmic.200700460

Vancouver

Wulff T, Jessen F, Roepstorff P, Hoffmann EK. Long term anoxia in rainbow trout investigated by 2-DE and MS/MS. Proteomics. 2008;1009 - 1018. https://doi.org/10.1002/pmic.200700460

Author

Wulff, Tune ; Jessen, Flemming ; Roepstorff, Peter ; Hoffmann, Else Kay. / Long term anoxia in rainbow trout investigated by 2-DE and MS/MS. In: Proteomics. 2008 ; pp. 1009 - 1018.

Bibtex

@article{7a26b920d65311dcbee902004c4f4f50,
title = "Long term anoxia in rainbow trout investigated by 2-DE and MS/MS.",
abstract = "Twenty-four hours of N(2) induced anoxia induced global perturbations on protein expression in rainbow trout hypodermal fibroblasts cell line. Anoxia was obtained by depleting the medium of O(2) by flushing with N(2), and protein changes were studied by 2-DE coupled with MS providing quantitative measurements of a large number of proteins in one single study. The anoxic insult changed the level of 33 protein spots: 22 of these were up-regulated compared to the control situation and 11 were down-regulated. Using MS/MS sequencing 19 of the 33 protein spots that changed were identified, corresponding to a success rate of more than 50%. The identified proteins included two proteins involved in energy metabolism namely phosphoglycerate mutase and isocitrate dehydrogenase. In addition we observed the up-regulation of a cluster of proteins that contribute to cytoskeleton function. These are calpain, EB1, and Rho GDP dissociation inhibitor (GDI). The up-regulation of Rho GDI was shown to develop in a time dependent manner with no significant increase for up to 8 h of anoxia. In conclusion, this study provides a thorough investigation of the effect of anoxia in a cell line from rainbow trout.",
author = "Tune Wulff and Flemming Jessen and Peter Roepstorff and Hoffmann, {Else Kay}",
note = "Keywords Anoxia • MS/MS • Rainbow trout • Two-dimensional gel electrophoresis",
year = "2008",
doi = "10.1002/pmic.200700460",
language = "English",
pages = "1009 -- 1018",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

}

RIS

TY - JOUR

T1 - Long term anoxia in rainbow trout investigated by 2-DE and MS/MS.

AU - Wulff, Tune

AU - Jessen, Flemming

AU - Roepstorff, Peter

AU - Hoffmann, Else Kay

N1 - Keywords Anoxia • MS/MS • Rainbow trout • Two-dimensional gel electrophoresis

PY - 2008

Y1 - 2008

N2 - Twenty-four hours of N(2) induced anoxia induced global perturbations on protein expression in rainbow trout hypodermal fibroblasts cell line. Anoxia was obtained by depleting the medium of O(2) by flushing with N(2), and protein changes were studied by 2-DE coupled with MS providing quantitative measurements of a large number of proteins in one single study. The anoxic insult changed the level of 33 protein spots: 22 of these were up-regulated compared to the control situation and 11 were down-regulated. Using MS/MS sequencing 19 of the 33 protein spots that changed were identified, corresponding to a success rate of more than 50%. The identified proteins included two proteins involved in energy metabolism namely phosphoglycerate mutase and isocitrate dehydrogenase. In addition we observed the up-regulation of a cluster of proteins that contribute to cytoskeleton function. These are calpain, EB1, and Rho GDP dissociation inhibitor (GDI). The up-regulation of Rho GDI was shown to develop in a time dependent manner with no significant increase for up to 8 h of anoxia. In conclusion, this study provides a thorough investigation of the effect of anoxia in a cell line from rainbow trout.

AB - Twenty-four hours of N(2) induced anoxia induced global perturbations on protein expression in rainbow trout hypodermal fibroblasts cell line. Anoxia was obtained by depleting the medium of O(2) by flushing with N(2), and protein changes were studied by 2-DE coupled with MS providing quantitative measurements of a large number of proteins in one single study. The anoxic insult changed the level of 33 protein spots: 22 of these were up-regulated compared to the control situation and 11 were down-regulated. Using MS/MS sequencing 19 of the 33 protein spots that changed were identified, corresponding to a success rate of more than 50%. The identified proteins included two proteins involved in energy metabolism namely phosphoglycerate mutase and isocitrate dehydrogenase. In addition we observed the up-regulation of a cluster of proteins that contribute to cytoskeleton function. These are calpain, EB1, and Rho GDP dissociation inhibitor (GDI). The up-regulation of Rho GDI was shown to develop in a time dependent manner with no significant increase for up to 8 h of anoxia. In conclusion, this study provides a thorough investigation of the effect of anoxia in a cell line from rainbow trout.

U2 - 10.1002/pmic.200700460

DO - 10.1002/pmic.200700460

M3 - Journal article

C2 - 18240135

SP - 1009

EP - 1018

JO - Proteomics

JF - Proteomics

SN - 1615-9853

ER -

ID: 2646152