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Transcriptome changes in STSV2-infected Sulfolobus islandicus REY15A undergoing continuous CRISPR spacer acquisition

Publikation: Forskning - peer reviewTidsskriftartikel

Carlos León Sobrino, W.P. Kot, Roger Antony Garrett

A transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich and basal media over a 6 day period. Spacer acquisition preceded strong host growth retardation, altered transcriptional activity of four different CRISPR-Cas modules and changes in viral copy numbers, and with significant differences in the two media. Transcript levels of proteins involved in the cell cycle were reduced, while those of DNA replication, DNA repair, transcriptional regulation, and some antitoxin-toxin pairs and transposases were unchanged or enhanced. Antisense RNAs were implicated in the transcriptional regulation of adaptation and interference modules of the type I-A CRISPR-Cas system and evidence was found for the occurrence of functional coordination between the single CRISPR-Cas adaptation module and the functionally diverse interference modules.
OriginalsprogEngelsk
TidsskriftMolecular Microbiology
Vol/bind99
Tidsskriftsnummer4
Sider (fra-til)719-728
Antal sider10
ISSN1365-2958
DOI
StatusUdgivet - 2016

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