BIO Seminar: Fritz Roth

Mapping environment-dependent protein networks and functional human sequence variation

Speaker: Professor Fritz Roth, University of Toronto, Canada

Host: Professor Kresten Lindorff-Larsen, Section for Biomolecular Sciences

Sequencing technology has enabled experimentation at scales that are often limited only by our ability to design multiplexing strategies. First, I will describe the Barcode Fusion Genetics (BFG) strategy for tracking all combinations of two barcoded libraries, and its use for proteome-scale mapping of interactions in yeast under multiple environmental conditions. Second, I will cover the TileSeq strategy for measuring the functional effects of nearly all possible missense variants in target proteins, and its potential to provide exhaustive variant effect maps for all human disease-associated proteins.