A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins

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Standard

A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins. / Bolla, Jani Reddy; Corey, Robin A.; Sahin, Cagla; Gault, Joseph; Hummer, Alissa; Hopper, Jonathan T. S.; Lane, David P.; Drew, David; Allison, Timothy M.; Stansfeld, Phillip J.; Robinson, Carol V.; Landreh, Michael.

I: Angewandte Chemie - International Edition, Bind 59, Nr. 9, 2020, s. 3523-3528.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Bolla, JR, Corey, RA, Sahin, C, Gault, J, Hummer, A, Hopper, JTS, Lane, DP, Drew, D, Allison, TM, Stansfeld, PJ, Robinson, CV & Landreh, M 2020, 'A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins', Angewandte Chemie - International Edition, bind 59, nr. 9, s. 3523-3528. https://doi.org/10.1002/anie.201914411

APA

Bolla, J. R., Corey, R. A., Sahin, C., Gault, J., Hummer, A., Hopper, J. T. S., Lane, D. P., Drew, D., Allison, T. M., Stansfeld, P. J., Robinson, C. V., & Landreh, M. (2020). A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins. Angewandte Chemie - International Edition, 59(9), 3523-3528. https://doi.org/10.1002/anie.201914411

Vancouver

Bolla JR, Corey RA, Sahin C, Gault J, Hummer A, Hopper JTS o.a. A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins. Angewandte Chemie - International Edition. 2020;59(9):3523-3528. https://doi.org/10.1002/anie.201914411

Author

Bolla, Jani Reddy ; Corey, Robin A. ; Sahin, Cagla ; Gault, Joseph ; Hummer, Alissa ; Hopper, Jonathan T. S. ; Lane, David P. ; Drew, David ; Allison, Timothy M. ; Stansfeld, Phillip J. ; Robinson, Carol V. ; Landreh, Michael. / A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins. I: Angewandte Chemie - International Edition. 2020 ; Bind 59, Nr. 9. s. 3523-3528.

Bibtex

@article{822fc2437bcf449b93784bd15cca187f,
title = "A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins",
abstract = "Membrane proteins engage in a variety of contacts with their surrounding lipids, but distinguishing between specifically bound lipids, and non-specific, annular interactions is a challenging problem. Applying native mass spectrometry to three membrane protein complexes with different lipid-binding properties, we explore the ability of detergents to compete with lipids bound in different environments. We show that lipids in annular positions on the presenilin homologue protease are subject to constant exchange with detergent. By contrast, detergent-resistant lipids bound at the dimer interface in the leucine transporter show decreased koff rates in molecular dynamics simulations. Turning to the lipid flippase MurJ, we find that addition of the natural substrate lipid-II results in the formation of a 1:1 protein–lipid complex, where the lipid cannot be displaced by detergent from the highly protected active site. In summary, we distinguish annular from non-annular lipids based on their exchange rates in solution.",
keywords = "lipid binding, membrane protein structure, molecular dynamics, native mass spectrometry",
author = "Bolla, {Jani Reddy} and Corey, {Robin A.} and Cagla Sahin and Joseph Gault and Alissa Hummer and Hopper, {Jonathan T. S.} and Lane, {David P.} and David Drew and Allison, {Timothy M.} and Stansfeld, {Phillip J.} and Robinson, {Carol V.} and Michael Landreh",
year = "2020",
doi = "10.1002/anie.201914411",
language = "English",
volume = "59",
pages = "3523--3528",
journal = "Angewandte Chemie International Edition",
issn = "1433-7851",
publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",
number = "9",

}

RIS

TY - JOUR

T1 - A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins

AU - Bolla, Jani Reddy

AU - Corey, Robin A.

AU - Sahin, Cagla

AU - Gault, Joseph

AU - Hummer, Alissa

AU - Hopper, Jonathan T. S.

AU - Lane, David P.

AU - Drew, David

AU - Allison, Timothy M.

AU - Stansfeld, Phillip J.

AU - Robinson, Carol V.

AU - Landreh, Michael

PY - 2020

Y1 - 2020

N2 - Membrane proteins engage in a variety of contacts with their surrounding lipids, but distinguishing between specifically bound lipids, and non-specific, annular interactions is a challenging problem. Applying native mass spectrometry to three membrane protein complexes with different lipid-binding properties, we explore the ability of detergents to compete with lipids bound in different environments. We show that lipids in annular positions on the presenilin homologue protease are subject to constant exchange with detergent. By contrast, detergent-resistant lipids bound at the dimer interface in the leucine transporter show decreased koff rates in molecular dynamics simulations. Turning to the lipid flippase MurJ, we find that addition of the natural substrate lipid-II results in the formation of a 1:1 protein–lipid complex, where the lipid cannot be displaced by detergent from the highly protected active site. In summary, we distinguish annular from non-annular lipids based on their exchange rates in solution.

AB - Membrane proteins engage in a variety of contacts with their surrounding lipids, but distinguishing between specifically bound lipids, and non-specific, annular interactions is a challenging problem. Applying native mass spectrometry to three membrane protein complexes with different lipid-binding properties, we explore the ability of detergents to compete with lipids bound in different environments. We show that lipids in annular positions on the presenilin homologue protease are subject to constant exchange with detergent. By contrast, detergent-resistant lipids bound at the dimer interface in the leucine transporter show decreased koff rates in molecular dynamics simulations. Turning to the lipid flippase MurJ, we find that addition of the natural substrate lipid-II results in the formation of a 1:1 protein–lipid complex, where the lipid cannot be displaced by detergent from the highly protected active site. In summary, we distinguish annular from non-annular lipids based on their exchange rates in solution.

KW - lipid binding

KW - membrane protein structure

KW - molecular dynamics

KW - native mass spectrometry

U2 - 10.1002/anie.201914411

DO - 10.1002/anie.201914411

M3 - Journal article

C2 - 31886601

AN - SCOPUS:85078676975

VL - 59

SP - 3523

EP - 3528

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

SN - 1433-7851

IS - 9

ER -

ID: 236316282