A strand specific high resolution normalization method for chip-sequencing data employing multiple experimental control measurements.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
Dokumenter
- A strand...
Forlagets udgivne version, 556 KB, PDF-dokument
High-throughput sequencing is becoming the standard tool for investigating protein-DNA interactions or epigenetic modifications. However, the data generated will always contain noise due to e.g. repetitive regions or non-specific antibody interactions. The noise will appear in the form of a background distribution of reads that must be taken into account in the downstream analysis, for example when detecting enriched regions (peak-calling). Several reported peak-callers can take experimental measurements of background tag distribution into account when analysing a data set. Unfortunately, the background is only used to adjust peak calling and not as a pre-processing step that aims at discerning the signal from the background noise. A normalization procedure that extracts the signal of interest would be of universal use when investigating genomic patterns.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Algorithms for Molecular Biology |
Vol/bind | 7 |
Udgave nummer | 1 |
Sider (fra-til) | 2 |
Antal sider | 10 |
ISSN | 1748-7188 |
DOI | |
Status | Udgivet - 2012 |
Antal downloads er baseret på statistik fra Google Scholar og www.ku.dk
Ingen data tilgængelig
ID: 43869460