DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features

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Standard

DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features. / Lin, Jinzhong; Fuglsang, Anders; Kjeldsen, Anders Lynge; Sun, Kaiyan; Bhoobalan-Chitty, Yuvaraj; Peng, Xu.

I: Nucleic Acids Research, Bind 48, Nr. 18, 2020, s. 10470-10478.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lin, J, Fuglsang, A, Kjeldsen, AL, Sun, K, Bhoobalan-Chitty, Y & Peng, X 2020, 'DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features', Nucleic Acids Research, bind 48, nr. 18, s. 10470-10478. https://doi.org/10.1093/nar/gkaa749

APA

Lin, J., Fuglsang, A., Kjeldsen, A. L., Sun, K., Bhoobalan-Chitty, Y., & Peng, X. (2020). DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features. Nucleic Acids Research, 48(18), 10470-10478. https://doi.org/10.1093/nar/gkaa749

Vancouver

Lin J, Fuglsang A, Kjeldsen AL, Sun K, Bhoobalan-Chitty Y, Peng X. DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features. Nucleic Acids Research. 2020;48(18):10470-10478. https://doi.org/10.1093/nar/gkaa749

Author

Lin, Jinzhong ; Fuglsang, Anders ; Kjeldsen, Anders Lynge ; Sun, Kaiyan ; Bhoobalan-Chitty, Yuvaraj ; Peng, Xu. / DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features. I: Nucleic Acids Research. 2020 ; Bind 48, Nr. 18. s. 10470-10478.

Bibtex

@article{82fb8b1380444574bc5fbbdf01fb8522,
title = "DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features",
abstract = "Prokaryotic CRISPR-Cas immune systems are classified into six types based on their effector complexes which cleave dsDNA specifically (types I, II and V), ssRNA exclusively (type VI) or both ssRNA via a ruler mechanism and ssDNA unspecifically (type III). To date, no specific cleavage of ssDNA target has been reported for CRISPR-Cas. Here, we demonstrate dual dsDNA and ssDNA cleavage activities of a subtype I-D system which carries a type III Cas10-like large subunit, Cas10d. In addition to a specific dsDNA cleavage activity dependent on the HD domain of Cas10d, the helicase Cas3' and a compatible protospacer adjacent motif (PAM), the subtype I-D effector complex can cleave ssDNA that is complementary in sequence to the crRNA. Significantly, the ssDNA cleavage sites occur at 6-nt intervals and the cleavage is catalysed by the backbone subunit Csc2 (Cas7), similar to the periodic cleavage of ssRNA by the backbone subunit of type III effectors. The typical type I cleavage of dsDNA combined with the exceptional 6-nt spaced cleavage of ssDNA and the presence of a type III like large subunit provide strong evidence for the subtype I-D system being an evolutionary intermediate between type I and type III CRISPR-Cas systems.",
author = "Jinzhong Lin and Anders Fuglsang and Kjeldsen, {Anders Lynge} and Kaiyan Sun and Yuvaraj Bhoobalan-Chitty and Xu Peng",
year = "2020",
doi = "10.1093/nar/gkaa749",
language = "English",
volume = "48",
pages = "10470--10478",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "18",

}

RIS

TY - JOUR

T1 - DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features

AU - Lin, Jinzhong

AU - Fuglsang, Anders

AU - Kjeldsen, Anders Lynge

AU - Sun, Kaiyan

AU - Bhoobalan-Chitty, Yuvaraj

AU - Peng, Xu

PY - 2020

Y1 - 2020

N2 - Prokaryotic CRISPR-Cas immune systems are classified into six types based on their effector complexes which cleave dsDNA specifically (types I, II and V), ssRNA exclusively (type VI) or both ssRNA via a ruler mechanism and ssDNA unspecifically (type III). To date, no specific cleavage of ssDNA target has been reported for CRISPR-Cas. Here, we demonstrate dual dsDNA and ssDNA cleavage activities of a subtype I-D system which carries a type III Cas10-like large subunit, Cas10d. In addition to a specific dsDNA cleavage activity dependent on the HD domain of Cas10d, the helicase Cas3' and a compatible protospacer adjacent motif (PAM), the subtype I-D effector complex can cleave ssDNA that is complementary in sequence to the crRNA. Significantly, the ssDNA cleavage sites occur at 6-nt intervals and the cleavage is catalysed by the backbone subunit Csc2 (Cas7), similar to the periodic cleavage of ssRNA by the backbone subunit of type III effectors. The typical type I cleavage of dsDNA combined with the exceptional 6-nt spaced cleavage of ssDNA and the presence of a type III like large subunit provide strong evidence for the subtype I-D system being an evolutionary intermediate between type I and type III CRISPR-Cas systems.

AB - Prokaryotic CRISPR-Cas immune systems are classified into six types based on their effector complexes which cleave dsDNA specifically (types I, II and V), ssRNA exclusively (type VI) or both ssRNA via a ruler mechanism and ssDNA unspecifically (type III). To date, no specific cleavage of ssDNA target has been reported for CRISPR-Cas. Here, we demonstrate dual dsDNA and ssDNA cleavage activities of a subtype I-D system which carries a type III Cas10-like large subunit, Cas10d. In addition to a specific dsDNA cleavage activity dependent on the HD domain of Cas10d, the helicase Cas3' and a compatible protospacer adjacent motif (PAM), the subtype I-D effector complex can cleave ssDNA that is complementary in sequence to the crRNA. Significantly, the ssDNA cleavage sites occur at 6-nt intervals and the cleavage is catalysed by the backbone subunit Csc2 (Cas7), similar to the periodic cleavage of ssRNA by the backbone subunit of type III effectors. The typical type I cleavage of dsDNA combined with the exceptional 6-nt spaced cleavage of ssDNA and the presence of a type III like large subunit provide strong evidence for the subtype I-D system being an evolutionary intermediate between type I and type III CRISPR-Cas systems.

U2 - 10.1093/nar/gkaa749

DO - 10.1093/nar/gkaa749

M3 - Journal article

C2 - 32960267

AN - SCOPUS:85092749651

VL - 48

SP - 10470

EP - 10478

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 18

ER -

ID: 250916613