Functional characterization of the vertebrate primary ureter: Structure and ion transport mechanisms of the pronephric duct in axolotl larvae (Amphibia)

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Functional characterization of the vertebrate primary ureter: Structure and ion transport mechanisms of the pronephric duct in axolotl larvae (Amphibia). / Haugan, Birgitte M; Halberg, Kenneth Agerlin; Jespersen, Åse; Prehn, Lea R; Møbjerg, Nadja.

I: BMC Developmental Biology, Bind 10, Nr. 56, 2010.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Haugan, BM, Halberg, KA, Jespersen, Å, Prehn, LR & Møbjerg, N 2010, 'Functional characterization of the vertebrate primary ureter: Structure and ion transport mechanisms of the pronephric duct in axolotl larvae (Amphibia)', BMC Developmental Biology, bind 10, nr. 56. https://doi.org/10.1186/1471-213X-10-56

APA

Haugan, B. M., Halberg, K. A., Jespersen, Å., Prehn, L. R., & Møbjerg, N. (2010). Functional characterization of the vertebrate primary ureter: Structure and ion transport mechanisms of the pronephric duct in axolotl larvae (Amphibia). BMC Developmental Biology, 10(56). https://doi.org/10.1186/1471-213X-10-56

Vancouver

Haugan BM, Halberg KA, Jespersen Å, Prehn LR, Møbjerg N. Functional characterization of the vertebrate primary ureter: Structure and ion transport mechanisms of the pronephric duct in axolotl larvae (Amphibia). BMC Developmental Biology. 2010;10(56). https://doi.org/10.1186/1471-213X-10-56

Author

Haugan, Birgitte M ; Halberg, Kenneth Agerlin ; Jespersen, Åse ; Prehn, Lea R ; Møbjerg, Nadja. / Functional characterization of the vertebrate primary ureter: Structure and ion transport mechanisms of the pronephric duct in axolotl larvae (Amphibia). I: BMC Developmental Biology. 2010 ; Bind 10, Nr. 56.

Bibtex

@article{e09647f08e4f11df928f000ea68e967b,
title = "Functional characterization of the vertebrate primary ureter: Structure and ion transport mechanisms of the pronephric duct in axolotl larvae (Amphibia)",
abstract = "BackgroundThree kidney systems appear during vertebrate development: the pronephroi, mesonephroi and metanephroi. The pronephric duct is the first or primary ureter of these kidney systems. Its role as a key player in the induction of nephrogenic mesenchyme is well established. Here we investigate whether the duct is involved in urine modification using larvae of the freshwater amphibian Ambystoma mexicanum (axolotl) as model.ResultsWe investigated structural as well as physiological properties of the pronephric duct. The key elements of our methodology were: using histology, light and transmission electron microscopy as well as confocal laser scanning microscopy on fixed tissue and applying the microperfusion technique on isolated pronephric ducts in combination with single cell microelectrode impalements. Our data show that the fully differentiated pronephric duct is composed of a single layered epithelium consisting of one cell type comparable to the principal cell of the renal collecting duct system. The cells are characterized by a prominent basolateral labyrinth and a relatively smooth apical surface with one central cilium. Cellular impalements demonstrate the presence of apical Na+ and K+ conductances, as well as a large K+ conductance in the basolateral cell membrane. Immunolabeling experiments indicate heavy expression of Na+/K+-ATPase in the basolateral labyrinth.ConclusionsWe propose that the pronephric duct is important for the subsequent modification of urine produced by the pronephros. Our results indicate that it reabsorbs sodium and secretes potassium via channels present in the apical cell membrane with the driving force for ion movement provided by the Na+/K+ pump. This is to our knowledge the first characterization of the pronephric duct, the precursor of the collecting duct system, which provides a model of cell structure and basic mechanisms for ion transport. Such information may be important in understanding the evolution of vertebrate kidney systems and human diseases associated with congenital malformations.",
author = "Haugan, {Birgitte M} and Halberg, {Kenneth Agerlin} and {\AA}se Jespersen and Prehn, {Lea R} and Nadja M{\o}bjerg",
year = "2010",
doi = "10.1186/1471-213X-10-56",
language = "English",
volume = "10",
journal = "B M C Developmental Biology",
issn = "1471-213X",
publisher = "BioMed Central Ltd.",
number = "56",

}

RIS

TY - JOUR

T1 - Functional characterization of the vertebrate primary ureter: Structure and ion transport mechanisms of the pronephric duct in axolotl larvae (Amphibia)

AU - Haugan, Birgitte M

AU - Halberg, Kenneth Agerlin

AU - Jespersen, Åse

AU - Prehn, Lea R

AU - Møbjerg, Nadja

PY - 2010

Y1 - 2010

N2 - BackgroundThree kidney systems appear during vertebrate development: the pronephroi, mesonephroi and metanephroi. The pronephric duct is the first or primary ureter of these kidney systems. Its role as a key player in the induction of nephrogenic mesenchyme is well established. Here we investigate whether the duct is involved in urine modification using larvae of the freshwater amphibian Ambystoma mexicanum (axolotl) as model.ResultsWe investigated structural as well as physiological properties of the pronephric duct. The key elements of our methodology were: using histology, light and transmission electron microscopy as well as confocal laser scanning microscopy on fixed tissue and applying the microperfusion technique on isolated pronephric ducts in combination with single cell microelectrode impalements. Our data show that the fully differentiated pronephric duct is composed of a single layered epithelium consisting of one cell type comparable to the principal cell of the renal collecting duct system. The cells are characterized by a prominent basolateral labyrinth and a relatively smooth apical surface with one central cilium. Cellular impalements demonstrate the presence of apical Na+ and K+ conductances, as well as a large K+ conductance in the basolateral cell membrane. Immunolabeling experiments indicate heavy expression of Na+/K+-ATPase in the basolateral labyrinth.ConclusionsWe propose that the pronephric duct is important for the subsequent modification of urine produced by the pronephros. Our results indicate that it reabsorbs sodium and secretes potassium via channels present in the apical cell membrane with the driving force for ion movement provided by the Na+/K+ pump. This is to our knowledge the first characterization of the pronephric duct, the precursor of the collecting duct system, which provides a model of cell structure and basic mechanisms for ion transport. Such information may be important in understanding the evolution of vertebrate kidney systems and human diseases associated with congenital malformations.

AB - BackgroundThree kidney systems appear during vertebrate development: the pronephroi, mesonephroi and metanephroi. The pronephric duct is the first or primary ureter of these kidney systems. Its role as a key player in the induction of nephrogenic mesenchyme is well established. Here we investigate whether the duct is involved in urine modification using larvae of the freshwater amphibian Ambystoma mexicanum (axolotl) as model.ResultsWe investigated structural as well as physiological properties of the pronephric duct. The key elements of our methodology were: using histology, light and transmission electron microscopy as well as confocal laser scanning microscopy on fixed tissue and applying the microperfusion technique on isolated pronephric ducts in combination with single cell microelectrode impalements. Our data show that the fully differentiated pronephric duct is composed of a single layered epithelium consisting of one cell type comparable to the principal cell of the renal collecting duct system. The cells are characterized by a prominent basolateral labyrinth and a relatively smooth apical surface with one central cilium. Cellular impalements demonstrate the presence of apical Na+ and K+ conductances, as well as a large K+ conductance in the basolateral cell membrane. Immunolabeling experiments indicate heavy expression of Na+/K+-ATPase in the basolateral labyrinth.ConclusionsWe propose that the pronephric duct is important for the subsequent modification of urine produced by the pronephros. Our results indicate that it reabsorbs sodium and secretes potassium via channels present in the apical cell membrane with the driving force for ion movement provided by the Na+/K+ pump. This is to our knowledge the first characterization of the pronephric duct, the precursor of the collecting duct system, which provides a model of cell structure and basic mechanisms for ion transport. Such information may be important in understanding the evolution of vertebrate kidney systems and human diseases associated with congenital malformations.

U2 - 10.1186/1471-213X-10-56

DO - 10.1186/1471-213X-10-56

M3 - Journal article

C2 - 20507566

VL - 10

JO - B M C Developmental Biology

JF - B M C Developmental Biology

SN - 1471-213X

IS - 56

ER -

ID: 20806973