High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging
Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt
Dokumenter
- High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging
Forlagets udgivne version, 1,01 MB, PDF-dokument
The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.
Originalsprog | Engelsk |
---|---|
Artikelnummer | 106343 |
Tidsskrift | Journal of Microbiological Methods |
Vol/bind | 190 |
Antal sider | 5 |
ISSN | 0167-7012 |
DOI | |
Status | Udgivet - 2021 |
Bibliografisk note
Copyright © 2021. Published by Elsevier B.V.
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