Hypoxia-induced retinopathy model in adult zebrafish

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Hypoxia-induced retinopathy model in adult zebrafish. / Cao, Ziquan; Jensen, Lasse D.; Rouhi, Pegah; Hosaka, Kayoko; Lanne, Toste; Steffensen, John Fleng; Wahlberg, Erik; Cao, Yihai.

I: Nature Protocols (Print), Bind 5, Nr. 12, 01.12.2010, s. 1903-1910.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Cao, Z, Jensen, LD, Rouhi, P, Hosaka, K, Lanne, T, Steffensen, JF, Wahlberg, E & Cao, Y 2010, 'Hypoxia-induced retinopathy model in adult zebrafish', Nature Protocols (Print), bind 5, nr. 12, s. 1903-1910. https://doi.org/10.1038/nprot.2010.149

APA

Cao, Z., Jensen, L. D., Rouhi, P., Hosaka, K., Lanne, T., Steffensen, J. F., Wahlberg, E., & Cao, Y. (2010). Hypoxia-induced retinopathy model in adult zebrafish. Nature Protocols (Print), 5(12), 1903-1910. https://doi.org/10.1038/nprot.2010.149

Vancouver

Cao Z, Jensen LD, Rouhi P, Hosaka K, Lanne T, Steffensen JF o.a. Hypoxia-induced retinopathy model in adult zebrafish. Nature Protocols (Print). 2010 dec. 1;5(12):1903-1910. https://doi.org/10.1038/nprot.2010.149

Author

Cao, Ziquan ; Jensen, Lasse D. ; Rouhi, Pegah ; Hosaka, Kayoko ; Lanne, Toste ; Steffensen, John Fleng ; Wahlberg, Erik ; Cao, Yihai. / Hypoxia-induced retinopathy model in adult zebrafish. I: Nature Protocols (Print). 2010 ; Bind 5, Nr. 12. s. 1903-1910.

Bibtex

@article{769f12016f854b33a86b0bda00f7b101,
title = "Hypoxia-induced retinopathy model in adult zebrafish",
abstract = "Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1: EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.",
author = "Ziquan Cao and Jensen, {Lasse D.} and Pegah Rouhi and Kayoko Hosaka and Toste Lanne and Steffensen, {John Fleng} and Erik Wahlberg and Yihai Cao",
year = "2010",
month = dec,
day = "1",
doi = "10.1038/nprot.2010.149",
language = "English",
volume = "5",
pages = "1903--1910",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "nature publishing group",
number = "12",

}

RIS

TY - JOUR

T1 - Hypoxia-induced retinopathy model in adult zebrafish

AU - Cao, Ziquan

AU - Jensen, Lasse D.

AU - Rouhi, Pegah

AU - Hosaka, Kayoko

AU - Lanne, Toste

AU - Steffensen, John Fleng

AU - Wahlberg, Erik

AU - Cao, Yihai

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1: EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.

AB - Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1: EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.

U2 - 10.1038/nprot.2010.149

DO - 10.1038/nprot.2010.149

M3 - Journal article

C2 - 21127484

VL - 5

SP - 1903

EP - 1910

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 12

ER -

ID: 34358901