Hypoxia-induced retinopathy model in adult zebrafish
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
Standard
Hypoxia-induced retinopathy model in adult zebrafish. / Cao, Ziquan; Jensen, Lasse D.; Rouhi, Pegah; Hosaka, Kayoko; Lanne, Toste; Steffensen, John Fleng; Wahlberg, Erik; Cao, Yihai.
I: Nature Protocols (Print), Bind 5, Nr. 12, 01.12.2010, s. 1903-1910.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Hypoxia-induced retinopathy model in adult zebrafish
AU - Cao, Ziquan
AU - Jensen, Lasse D.
AU - Rouhi, Pegah
AU - Hosaka, Kayoko
AU - Lanne, Toste
AU - Steffensen, John Fleng
AU - Wahlberg, Erik
AU - Cao, Yihai
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1: EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.
AB - Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1: EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs.
U2 - 10.1038/nprot.2010.149
DO - 10.1038/nprot.2010.149
M3 - Journal article
C2 - 21127484
VL - 5
SP - 1903
EP - 1910
JO - Nature Protocols
JF - Nature Protocols
SN - 1754-2189
IS - 12
ER -
ID: 34358901