MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.
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MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. / Düring, Louis; Thorsen, Michael; Petersen, Darima; Køster, Brian; Jensen, Torben Heick; Holmberg, Steen.
I: P L o S One, Bind 7, Nr. 9, 2012.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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T1 - MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.
AU - Düring, Louis
AU - Thorsen, Michael
AU - Petersen, Darima
AU - Køster, Brian
AU - Jensen, Torben Heick
AU - Holmberg, Steen
PY - 2012
Y1 - 2012
N2 - A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6¿¿ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6¿¿ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6¿¿ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6¿¿) as a growth suppressor of rsc nhp6¿¿ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309¿, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.
AB - A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6¿¿ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6¿¿ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6¿¿ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6¿¿) as a growth suppressor of rsc nhp6¿¿ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309¿, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.
U2 - 10.1371/journal.pone.0044373
DO - 10.1371/journal.pone.0044373
M3 - Journal article
C2 - 23028530
VL - 7
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 9
ER -
ID: 44688886