MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.

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Standard

MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. / Düring, Louis; Thorsen, Michael; Petersen, Darima; Køster, Brian; Jensen, Torben Heick; Holmberg, Steen.

I: P L o S One, Bind 7, Nr. 9, 2012.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Düring, L, Thorsen, M, Petersen, D, Køster, B, Jensen, TH & Holmberg, S 2012, 'MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.', P L o S One, bind 7, nr. 9. https://doi.org/10.1371/journal.pone.0044373

APA

Düring, L., Thorsen, M., Petersen, D., Køster, B., Jensen, T. H., & Holmberg, S. (2012). MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. P L o S One, 7(9). https://doi.org/10.1371/journal.pone.0044373

Vancouver

Düring L, Thorsen M, Petersen D, Køster B, Jensen TH, Holmberg S. MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. P L o S One. 2012;7(9). https://doi.org/10.1371/journal.pone.0044373

Author

Düring, Louis ; Thorsen, Michael ; Petersen, Darima ; Køster, Brian ; Jensen, Torben Heick ; Holmberg, Steen. / MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. I: P L o S One. 2012 ; Bind 7, Nr. 9.

Bibtex

@article{3b46885b33cd47d3bfa6d09f38b52615,
title = "MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.",
abstract = "A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6¿¿ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6¿¿ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6¿¿ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6¿¿) as a growth suppressor of rsc nhp6¿¿ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309¿, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.",
author = "Louis D{\"u}ring and Michael Thorsen and Darima Petersen and Brian K{\o}ster and Jensen, {Torben Heick} and Steen Holmberg",
year = "2012",
doi = "10.1371/journal.pone.0044373",
language = "English",
volume = "7",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.

AU - Düring, Louis

AU - Thorsen, Michael

AU - Petersen, Darima

AU - Køster, Brian

AU - Jensen, Torben Heick

AU - Holmberg, Steen

PY - 2012

Y1 - 2012

N2 - A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6¿¿ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6¿¿ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6¿¿ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6¿¿) as a growth suppressor of rsc nhp6¿¿ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309¿, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.

AB - A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6¿¿ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6¿¿ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6¿¿ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6¿¿) as a growth suppressor of rsc nhp6¿¿ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309¿, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.

U2 - 10.1371/journal.pone.0044373

DO - 10.1371/journal.pone.0044373

M3 - Journal article

C2 - 23028530

VL - 7

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 9

ER -

ID: 44688886