MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Dokumenter

  • Louis Düring
  • Michael Thorsen
  • Darima Petersen
  • Brian Køster
  • Torben Heick Jensen
  • Holmberg, Steen
A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6¿¿ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6¿¿ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6¿¿ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6¿¿) as a growth suppressor of rsc nhp6¿¿ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309¿, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.
OriginalsprogEngelsk
TidsskriftP L o S One
Vol/bind7
Udgave nummer9
Antal sider13
ISSN1932-6203
DOI
StatusUdgivet - 2012

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