Predicting the Shapes of Protein Complexes through Collision Cross Section Measurements and Database Searches

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  • Michael Landreh
  • Sahin, Cagla
  • Joseph Gault
  • Samira Sadeghi
  • Chester L. Drum
  • Povilas Uzdavinys
  • David Drew
  • Timothy M. Allison
  • Matteo T. Degiacomi
  • Erik G. Marklund

In structural biology, collision cross sections (CCSs) from ion mobility mass spectrometry (IM-MS) measurements are routinely compared to computationally or experimentally derived protein structures. Here, we investigate whether CCS data can inform about the shape of a protein in the absence of specific reference structures. Analysis of the proteins in the CCS database shows that protein complexes with low apparent densities are structurally more diverse than those with a high apparent density. Although assigning protein shapes purely on CCS data is not possible, we find that we can distinguish oblate- and prolate-shaped protein complexes by using the CCS, molecular weight, and oligomeric states to mine the Protein Data Bank (PDB) for potentially similar protein structures. Furthermore, comparing the CCS of a ferritin cage to the solution structures in the PDB reveals significant deviations caused by structural collapse in the gas phase. We then apply the strategy to an integral membrane protein by comparing the shapes of a prokaryotic and a eukaryotic sodium/proton antiporter homologue. We conclude that mining the PDB with IM-MS data is a time-effective way to derive low-resolution structural models.

OriginalsprogEngelsk
TidsskriftAnalytical Chemistry
Vol/bind92
Udgave nummer18
Sider (fra-til)12297-12303
Antal sider7
ISSN0003-2700
DOI
StatusUdgivet - 2020

ID: 249908692