Resistance and Vulnerability of Honeybee (Apis mellifera) Gut Bacteria to Commonly Used Pesticides

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Resistance and Vulnerability of Honeybee (Apis mellifera) Gut Bacteria to Commonly Used Pesticides. / Cuesta-Maté, Ana; Renelies-Hamilton, Justinn; Kryger, Per; Jensen, Annette Bruun; Sinotte, Veronica M.; Poulsen, Michael.

I: Frontiers in Microbiology, Bind 12, 717990, 2021.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Cuesta-Maté, A, Renelies-Hamilton, J, Kryger, P, Jensen, AB, Sinotte, VM & Poulsen, M 2021, 'Resistance and Vulnerability of Honeybee (Apis mellifera) Gut Bacteria to Commonly Used Pesticides', Frontiers in Microbiology, bind 12, 717990. https://doi.org/10.3389/fmicb.2021.717990

APA

Cuesta-Maté, A., Renelies-Hamilton, J., Kryger, P., Jensen, A. B., Sinotte, V. M., & Poulsen, M. (2021). Resistance and Vulnerability of Honeybee (Apis mellifera) Gut Bacteria to Commonly Used Pesticides. Frontiers in Microbiology, 12, [717990]. https://doi.org/10.3389/fmicb.2021.717990

Vancouver

Cuesta-Maté A, Renelies-Hamilton J, Kryger P, Jensen AB, Sinotte VM, Poulsen M. Resistance and Vulnerability of Honeybee (Apis mellifera) Gut Bacteria to Commonly Used Pesticides. Frontiers in Microbiology. 2021;12. 717990. https://doi.org/10.3389/fmicb.2021.717990

Author

Cuesta-Maté, Ana ; Renelies-Hamilton, Justinn ; Kryger, Per ; Jensen, Annette Bruun ; Sinotte, Veronica M. ; Poulsen, Michael. / Resistance and Vulnerability of Honeybee (Apis mellifera) Gut Bacteria to Commonly Used Pesticides. I: Frontiers in Microbiology. 2021 ; Bind 12.

Bibtex

@article{6a6f3c6fe62a4ae2a7d998eae6f62973,
title = "Resistance and Vulnerability of Honeybee (Apis mellifera) Gut Bacteria to Commonly Used Pesticides",
abstract = "Agricultural and apicultural practices expose honeybees to a range of pesticides that have the potential to negatively affect their physiology, neurobiology, and behavior. Accumulating evidence suggests that these effects extend to the honeybee gut microbiome, which serves important functions for honeybee health. Here we test the potential effects of the pesticides thiacloprid, acetamiprid, and oxalic acid on the gut microbiota of honeybees, first in direct in vitro inhibition assays and secondly in an in vivo caged bee experiment to test if exposure leads to gut microbiota community changes. We found that thiacloprid did not inhibit the honeybee core gut bacteria in vitro, nor did it affect overall community composition or richness in vivo. Acetamiprid did also not inhibit bacterial growth in vitro, but it did affect community structure within bees. The eight bacterial genera tested showed variable levels of susceptibility to oxalic acid in vitro. In vivo, treatment with this pesticide reduced amplicon sequence variant (ASV) richness and affected gut microbiome composition, with most marked impact on the common crop bacteria Lactobacillus kunkeei and the genus Bombella. We conducted network analyses which captured known associations between bacterial members and illustrated the sensitivity of the microbiome to environmental stressors. Our findings point to risks of honeybee exposure to oxalic acid, which has been deemed safe for use in treatment against Varroa mites in honeybee colonies, and we advocate for more extensive assessment of the long-term effects that it may have on honeybee health.",
keywords = "acaricide, anthropogenic stressor, microbiome, neonicotinoid, social insect, symbiosis",
author = "Ana Cuesta-Mat{\'e} and Justinn Renelies-Hamilton and Per Kryger and Jensen, {Annette Bruun} and Sinotte, {Veronica M.} and Michael Poulsen",
note = "Funding Information: We thank Philipp Engel for providing bacterial strains, Kasun H. Bodawatta for assistance in preparing samples for sequencing, and Suzanne Schmidt for ChemDraw structures included in Figure 1. Further, we thank the Social and Symbiotic Evolution Group for discussion of the project and for comments on the manuscript. Funding. This research was supported by a Ph.D. fellowship and research stipend from the Department of Biology, University of Copenhagen to VS and a European Research Council Consolidator Grant (771349) to MP. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Cuesta-Mat{\'e}, Renelies-Hamilton, Kryger, Jensen, Sinotte and Poulsen.",
year = "2021",
doi = "10.3389/fmicb.2021.717990",
language = "English",
volume = "12",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Resistance and Vulnerability of Honeybee (Apis mellifera) Gut Bacteria to Commonly Used Pesticides

AU - Cuesta-Maté, Ana

AU - Renelies-Hamilton, Justinn

AU - Kryger, Per

AU - Jensen, Annette Bruun

AU - Sinotte, Veronica M.

AU - Poulsen, Michael

N1 - Funding Information: We thank Philipp Engel for providing bacterial strains, Kasun H. Bodawatta for assistance in preparing samples for sequencing, and Suzanne Schmidt for ChemDraw structures included in Figure 1. Further, we thank the Social and Symbiotic Evolution Group for discussion of the project and for comments on the manuscript. Funding. This research was supported by a Ph.D. fellowship and research stipend from the Department of Biology, University of Copenhagen to VS and a European Research Council Consolidator Grant (771349) to MP. Publisher Copyright: © Copyright © 2021 Cuesta-Maté, Renelies-Hamilton, Kryger, Jensen, Sinotte and Poulsen.

PY - 2021

Y1 - 2021

N2 - Agricultural and apicultural practices expose honeybees to a range of pesticides that have the potential to negatively affect their physiology, neurobiology, and behavior. Accumulating evidence suggests that these effects extend to the honeybee gut microbiome, which serves important functions for honeybee health. Here we test the potential effects of the pesticides thiacloprid, acetamiprid, and oxalic acid on the gut microbiota of honeybees, first in direct in vitro inhibition assays and secondly in an in vivo caged bee experiment to test if exposure leads to gut microbiota community changes. We found that thiacloprid did not inhibit the honeybee core gut bacteria in vitro, nor did it affect overall community composition or richness in vivo. Acetamiprid did also not inhibit bacterial growth in vitro, but it did affect community structure within bees. The eight bacterial genera tested showed variable levels of susceptibility to oxalic acid in vitro. In vivo, treatment with this pesticide reduced amplicon sequence variant (ASV) richness and affected gut microbiome composition, with most marked impact on the common crop bacteria Lactobacillus kunkeei and the genus Bombella. We conducted network analyses which captured known associations between bacterial members and illustrated the sensitivity of the microbiome to environmental stressors. Our findings point to risks of honeybee exposure to oxalic acid, which has been deemed safe for use in treatment against Varroa mites in honeybee colonies, and we advocate for more extensive assessment of the long-term effects that it may have on honeybee health.

AB - Agricultural and apicultural practices expose honeybees to a range of pesticides that have the potential to negatively affect their physiology, neurobiology, and behavior. Accumulating evidence suggests that these effects extend to the honeybee gut microbiome, which serves important functions for honeybee health. Here we test the potential effects of the pesticides thiacloprid, acetamiprid, and oxalic acid on the gut microbiota of honeybees, first in direct in vitro inhibition assays and secondly in an in vivo caged bee experiment to test if exposure leads to gut microbiota community changes. We found that thiacloprid did not inhibit the honeybee core gut bacteria in vitro, nor did it affect overall community composition or richness in vivo. Acetamiprid did also not inhibit bacterial growth in vitro, but it did affect community structure within bees. The eight bacterial genera tested showed variable levels of susceptibility to oxalic acid in vitro. In vivo, treatment with this pesticide reduced amplicon sequence variant (ASV) richness and affected gut microbiome composition, with most marked impact on the common crop bacteria Lactobacillus kunkeei and the genus Bombella. We conducted network analyses which captured known associations between bacterial members and illustrated the sensitivity of the microbiome to environmental stressors. Our findings point to risks of honeybee exposure to oxalic acid, which has been deemed safe for use in treatment against Varroa mites in honeybee colonies, and we advocate for more extensive assessment of the long-term effects that it may have on honeybee health.

KW - acaricide

KW - anthropogenic stressor

KW - microbiome

KW - neonicotinoid

KW - social insect

KW - symbiosis

U2 - 10.3389/fmicb.2021.717990

DO - 10.3389/fmicb.2021.717990

M3 - Journal article

C2 - 34539609

AN - SCOPUS:85115175472

VL - 12

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 717990

ER -

ID: 280618024