Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

  • Guoyi Dong
  • Zhouchun Shang
  • Longqi Liu
  • Chuanyu Liu
  • Yuping Ge
  • Quanlei Wang
  • Liang Wu
  • Fang Chen
  • Baolin Li
  • Xin Liu
  • Xun Xu
  • Huanming Yang
  • Yutao Du
  • Hui Jiang
Spermatogenic lineage has been directly generated in spermatogonial stem cell (SSC) conditions from human pluripotent stem cells (PSCs). However, it remains unknown whether mouse embryonic stem cells (ESCs) can directly differentiate into advanced male germ cell lineage in the same conditions. Here, we showed rather low efficiency of germ-like cell generation from mouse ESCs in SSC conditions. Interestingly, addition of retinoic acid (RA) into SSC conditions enabled efficient differentiation of mouse ESCs into germ-like cells, as shown by the activation of spermatogenesis-associated genes such as Mvh, Dazl, Prdm14, Stella, Scp1, Scp3, Stra8 and Rec8. In contrast, for cells cultured in control medium, the activation of the above genes barely occurred. In addition, RA with SSC conditions yielded colonies of Acrosin-expressing cells and the positive ratio reached a peak at day 6. Our work thus establishes a simple and cost-efficient approach for male germ like cell differentiation from mouse PSCs and may propose a useful strategy for studying spermatogenesis in vitro.
OriginalsprogEngelsk
ArtikelnummerBSR20170637
TidsskriftBioscience Reports
Vol/bind37
Udgave nummer2
Antal sider10
ISSN0144-8463
DOI
StatusUdgivet - 2017

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