Specificity of MYB interactions relies on motifs in ordered and disordered contexts

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Specificity of MYB interactions relies on motifs in ordered and disordered contexts. / Millard, Peter S.; Weber, Konrad; Kragelund, Birthe B.; Burow, Meike.

I: Nucleic Acids Research, Bind 47, Nr. 18, 2019, s. 9592-9608.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Millard, PS, Weber, K, Kragelund, BB & Burow, M 2019, 'Specificity of MYB interactions relies on motifs in ordered and disordered contexts', Nucleic Acids Research, bind 47, nr. 18, s. 9592-9608. https://doi.org/10.1093/nar/gkz691

APA

Millard, P. S., Weber, K., Kragelund, B. B., & Burow, M. (2019). Specificity of MYB interactions relies on motifs in ordered and disordered contexts. Nucleic Acids Research, 47(18), 9592-9608. https://doi.org/10.1093/nar/gkz691

Vancouver

Millard PS, Weber K, Kragelund BB, Burow M. Specificity of MYB interactions relies on motifs in ordered and disordered contexts. Nucleic Acids Research. 2019;47(18):9592-9608. https://doi.org/10.1093/nar/gkz691

Author

Millard, Peter S. ; Weber, Konrad ; Kragelund, Birthe B. ; Burow, Meike. / Specificity of MYB interactions relies on motifs in ordered and disordered contexts. I: Nucleic Acids Research. 2019 ; Bind 47, Nr. 18. s. 9592-9608.

Bibtex

@article{528e8e3aabb04abab71fe496473ab3c6,
title = "Specificity of MYB interactions relies on motifs in ordered and disordered contexts",
abstract = "Physical interactions between members of the MYB and bHLH transcription factor (TF) families regulate many important biological processes in plants. Not all reported MYB-bHLH interactions can be explained by the known binding sites in the R3 repeat of the MYB DNA-binding domain. Noteworthy, most of the sequence diversity of MYB TFs lies in their non-MYB regions, which contain orphan small subgroup-defining motifs not yet linked to molecular functions. Here, we identified the motif mediating interaction between MYB TFs from subgroup 12 and their bHLH partners. Unlike other known MYB-bHLH interactions, the motif locates to the centre of the predicted disordered non-MYB region. We characterised the core motif, which enabled accurate prediction of previously unknown bHLH-interacting MYB TFs in Arabidopsis thaliana, and we confirmed its functional importance in planta. Our results indicate a correlation between the MYB-bHLH interaction affinity and the phenotypic output controlled by the TF complex. The identification of an interaction motif outside R3 indicates that MYB-bHLH interactions must have arisen multiple times, independently and suggests many more motifs of functional relevance to be harvested from subgroup-specific studies.",
author = "Millard, {Peter S.} and Konrad Weber and Kragelund, {Birthe B.} and Meike Burow",
year = "2019",
doi = "10.1093/nar/gkz691",
language = "English",
volume = "47",
pages = "9592--9608",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "18",

}

RIS

TY - JOUR

T1 - Specificity of MYB interactions relies on motifs in ordered and disordered contexts

AU - Millard, Peter S.

AU - Weber, Konrad

AU - Kragelund, Birthe B.

AU - Burow, Meike

PY - 2019

Y1 - 2019

N2 - Physical interactions between members of the MYB and bHLH transcription factor (TF) families regulate many important biological processes in plants. Not all reported MYB-bHLH interactions can be explained by the known binding sites in the R3 repeat of the MYB DNA-binding domain. Noteworthy, most of the sequence diversity of MYB TFs lies in their non-MYB regions, which contain orphan small subgroup-defining motifs not yet linked to molecular functions. Here, we identified the motif mediating interaction between MYB TFs from subgroup 12 and their bHLH partners. Unlike other known MYB-bHLH interactions, the motif locates to the centre of the predicted disordered non-MYB region. We characterised the core motif, which enabled accurate prediction of previously unknown bHLH-interacting MYB TFs in Arabidopsis thaliana, and we confirmed its functional importance in planta. Our results indicate a correlation between the MYB-bHLH interaction affinity and the phenotypic output controlled by the TF complex. The identification of an interaction motif outside R3 indicates that MYB-bHLH interactions must have arisen multiple times, independently and suggests many more motifs of functional relevance to be harvested from subgroup-specific studies.

AB - Physical interactions between members of the MYB and bHLH transcription factor (TF) families regulate many important biological processes in plants. Not all reported MYB-bHLH interactions can be explained by the known binding sites in the R3 repeat of the MYB DNA-binding domain. Noteworthy, most of the sequence diversity of MYB TFs lies in their non-MYB regions, which contain orphan small subgroup-defining motifs not yet linked to molecular functions. Here, we identified the motif mediating interaction between MYB TFs from subgroup 12 and their bHLH partners. Unlike other known MYB-bHLH interactions, the motif locates to the centre of the predicted disordered non-MYB region. We characterised the core motif, which enabled accurate prediction of previously unknown bHLH-interacting MYB TFs in Arabidopsis thaliana, and we confirmed its functional importance in planta. Our results indicate a correlation between the MYB-bHLH interaction affinity and the phenotypic output controlled by the TF complex. The identification of an interaction motif outside R3 indicates that MYB-bHLH interactions must have arisen multiple times, independently and suggests many more motifs of functional relevance to be harvested from subgroup-specific studies.

U2 - 10.1093/nar/gkz691

DO - 10.1093/nar/gkz691

M3 - Journal article

C2 - 31400117

VL - 47

SP - 9592

EP - 9608

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 18

ER -

ID: 228411029