Synthesis of 1,2-phenylenediamine capturing molecule for the detection of diacetyl
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- Synthesis of 1,2-phenylenediamine capturing molecule for the detection of diacetyl
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Here we describe the design of 1,2-phenylenediamine capturing molecule and the synthesis steps necessary for its preparation. The designed 1,2-phenylenediamine derivative is able to capture diacetyl in solution, as shown by ESIMS, forming a chemical adduct, 1-4-quinoxaline. The methyl esters of diacetyl-adduct (DAA) and pentanedione-adduct (PDA) are incorporated to the lysines in BSA and the conjugate used for antibody screening and selection. In the research article is described an enzyme-linked immunosorbent assay developed to detect and quantify diacetyl in complex media.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Data in Brief |
Vol/bind | 15 |
Sider (fra-til) | 483-490 |
Antal sider | 8 |
ISSN | 2352-3409 |
DOI | |
Status | Udgivet - 2017 |
Eksternt udgivet | Ja |
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ID: 200826959