The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system. / Munk, Mads; Alcalde, Juan; Lorentzen, Lasse; Villalobo, Antonio; Berchtold, Martin W.; Panina, Svetlana.

I: Cell Calcium, Bind 88, 102207, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Munk, M, Alcalde, J, Lorentzen, L, Villalobo, A, Berchtold, MW & Panina, S 2020, 'The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system', Cell Calcium, bind 88, 102207. https://doi.org/10.1016/j.ceca.2020.102207

APA

Munk, M., Alcalde, J., Lorentzen, L., Villalobo, A., Berchtold, M. W., & Panina, S. (2020). The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system. Cell Calcium, 88, [102207]. https://doi.org/10.1016/j.ceca.2020.102207

Vancouver

Munk M, Alcalde J, Lorentzen L, Villalobo A, Berchtold MW, Panina S. The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system. Cell Calcium. 2020;88. 102207. https://doi.org/10.1016/j.ceca.2020.102207

Author

Munk, Mads ; Alcalde, Juan ; Lorentzen, Lasse ; Villalobo, Antonio ; Berchtold, Martin W. ; Panina, Svetlana. / The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system. I: Cell Calcium. 2020 ; Bind 88.

Bibtex

@article{e25a2cdbe5f640368f9419352ab58f8a,
title = "The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system",
abstract = "Calmodulin (CaM) is the principle mediator of the Ca2+ signal in all eukaryotic cells. A huge variety of basic cellular processes including cell cycle control, proliferation, secretion and motility, among many others are governed by CaM, which regulates activities of myriads of target proteins. Mammalian CaM is encoded by three genes localized on different chromosomes all producing an identical protein. In this study, we have generated HeLa human cancer cells conditionally expressing CaM in a genetic background with all three genes inactivated by CRISPR/Cas9. We demonstrate that downregulation of ectopically expressed CaM is achieved after 120 h, when cells are arrested in the M phase of the cell cycle. We show for the first time that CaM downregulation in human cancer cells is followed by a multinucleated senescent state as indicated by expression of β-galactosidase as well as cell morphology typical for senescent cells. Our newly generated genetic system may be useful for the analysis of other CaM regulated processes in eukaryotic cells in the absence of endogenous CaM genes.",
keywords = "Calmodulin, Cell cycle, CRISPR/Cas9, Mitotic arrest, Senescence, Tet-Off system",
author = "Mads Munk and Juan Alcalde and Lasse Lorentzen and Antonio Villalobo and Berchtold, {Martin W.} and Svetlana Panina",
year = "2020",
doi = "10.1016/j.ceca.2020.102207",
language = "English",
volume = "88",
journal = "Cell Calcium",
issn = "0143-4160",
publisher = "Churchill Livingstone",

}

RIS

TY - JOUR

T1 - The impact of calmodulin on the cell cycle analyzed in a novel human cellular genetic system

AU - Munk, Mads

AU - Alcalde, Juan

AU - Lorentzen, Lasse

AU - Villalobo, Antonio

AU - Berchtold, Martin W.

AU - Panina, Svetlana

PY - 2020

Y1 - 2020

N2 - Calmodulin (CaM) is the principle mediator of the Ca2+ signal in all eukaryotic cells. A huge variety of basic cellular processes including cell cycle control, proliferation, secretion and motility, among many others are governed by CaM, which regulates activities of myriads of target proteins. Mammalian CaM is encoded by three genes localized on different chromosomes all producing an identical protein. In this study, we have generated HeLa human cancer cells conditionally expressing CaM in a genetic background with all three genes inactivated by CRISPR/Cas9. We demonstrate that downregulation of ectopically expressed CaM is achieved after 120 h, when cells are arrested in the M phase of the cell cycle. We show for the first time that CaM downregulation in human cancer cells is followed by a multinucleated senescent state as indicated by expression of β-galactosidase as well as cell morphology typical for senescent cells. Our newly generated genetic system may be useful for the analysis of other CaM regulated processes in eukaryotic cells in the absence of endogenous CaM genes.

AB - Calmodulin (CaM) is the principle mediator of the Ca2+ signal in all eukaryotic cells. A huge variety of basic cellular processes including cell cycle control, proliferation, secretion and motility, among many others are governed by CaM, which regulates activities of myriads of target proteins. Mammalian CaM is encoded by three genes localized on different chromosomes all producing an identical protein. In this study, we have generated HeLa human cancer cells conditionally expressing CaM in a genetic background with all three genes inactivated by CRISPR/Cas9. We demonstrate that downregulation of ectopically expressed CaM is achieved after 120 h, when cells are arrested in the M phase of the cell cycle. We show for the first time that CaM downregulation in human cancer cells is followed by a multinucleated senescent state as indicated by expression of β-galactosidase as well as cell morphology typical for senescent cells. Our newly generated genetic system may be useful for the analysis of other CaM regulated processes in eukaryotic cells in the absence of endogenous CaM genes.

KW - Calmodulin

KW - Cell cycle

KW - CRISPR/Cas9

KW - Mitotic arrest

KW - Senescence

KW - Tet-Off system

U2 - 10.1016/j.ceca.2020.102207

DO - 10.1016/j.ceca.2020.102207

M3 - Journal article

C2 - 32408024

AN - SCOPUS:85084378839

VL - 88

JO - Cell Calcium

JF - Cell Calcium

SN - 0143-4160

M1 - 102207

ER -

ID: 244573348