Bacterial Growth Rate Regulation and RNA decay
|Main area:||Molecular biology|
|Target group:||Biology, Biochemistry, Molecular Biomedicine|
|Educational level:||Bachelor, Masters|
During growth in many cell types, translation is the most energy consuming process exceeding 50% of the total cellular energy budget. In the model organism Escherichia coli, we have found a very direct regulatory mechanism responsible for reducing the translational capacity of the cell during stress. The reduction occurs by degradation of important components of the translational machinery, namely the components made of RNA: the tRNAs and the ribosomal RNAs. The overall goal of the project is to identify the mechanisms behind degradation and understand how that can influence the fitness of bacteria in various situations.
|Methods used:||site-directed mutagenesis, Northern blot, measurements of macromolecular synthesis rates, RNA-sequencing, reporter gene fusions|
|Keywords:||tRNA, regulation of gene expression, Bacterial Stress Responses, protein synthesis, noncoding RNA|
|Supervisor(s):||Sine Lo Svenningsen and Michael A. Sørensen|