Designing new enzyme activities de novo

Main area:
Target group:Biochemistry, Biology
Educational level:Masters, Bachelor
Project description:

We are interested in developing new enzymes based on designed proteins. We employ a combination of genetic selection, semi randomized gene synthesis to computational design to select active enzymes. The project we initially mostly entail molecular biology and establishment of the genetic selection system. Later, protein purification and characterization will also be important.

Methods used:Molecular cloning and mutagenesis, heterologues expression and protein purification.
Project home page: http://www1.bio.ku.dk/english/research/bms/research/pbg/groups/jrw/research/
Supervisor(s): Jakob Winther
Email:jrwinther@bio.ku.dk