Molecular mechanisms behind growth optimization and stress responses in bacteria

Main area:Molecular biology
Target group:Biochemistry, Biology, Molecular Biomedicine
Educational level:Masters, Bachelor
Project description:

We are studying how bacteria sense diverse environmental parameters, like supplies of diverse nutrients, and how the regulatory signals are integrated to obtain the best survival strategy in the competition for supplies.

We use a lot of quantitative measurements in our studies to see where resources are spent in the cells and have recently found and published that ribosomes and tRNA are very unstable molecules in situations where translation becomes limited by substrate. We have used methods like quantitative high throughput RNA-seq, Northern blots and metabolic labeling with various radioactive precursers for these studies, and have by these methods gained insight into likely regulatory mechanisms involving small regulatory RNAs and specific RNases.

The current focus is to confirm these regulatory networks by molecular biology in genetically manipulated strains, either by deleting central genes or by doing over-expression/complementation studies with cloned genes.

Methods used:Northern blots, measurements on live cultures of reporters and macromolecules, bacterial genetics and clonings, qPCR and high thrugput seq.
Keywords:Regulatory networks, Growth physiology, Stress responses
Supervisor(s): Sine Lo Svenningsen and Michael A. Sørensen
Email:mas@bio.ku.dk