Comparative Genomic Analyses of Flavobacterium psychrophilum Isolates Reveals New Putative Genetic Determinants of Virulence Traits
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Comparative Genomic Analyses of Flavobacterium psychrophilum Isolates Reveals New Putative Genetic Determinants of Virulence Traits. / Castillo, Daniel; Donati, Valentina L.; Jørgensen, Jóhanna; Sundell, Krister; Dalsgaard, Inger; Madsen, Lone; Wiklund, Tom; Middelboe, Mathias.
In: Microorganisms, Vol. 9, No. 8, 1658, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Comparative Genomic Analyses of Flavobacterium psychrophilum Isolates Reveals New Putative Genetic Determinants of Virulence Traits
AU - Castillo, Daniel
AU - Donati, Valentina L.
AU - Jørgensen, Jóhanna
AU - Sundell, Krister
AU - Dalsgaard, Inger
AU - Madsen, Lone
AU - Wiklund, Tom
AU - Middelboe, Mathias
N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - The fish pathogen Flavobacterium psychrophilum is currently one of the main pathogenic bacteria hampering the productivity of salmonid farming worldwide. Although putative virulence determinants have been identified, the genetic basis for variation in virulence of F. psychrophilum is not fully understood. In this study, we analyzed whole-genome sequences of a collection of 25 F. psychrophilum isolates from Baltic Sea countries and compared genomic information with a previous determination of their virulence in juvenile rainbow trout. The results revealed a conserved population of F. psychrophilum that were consistently present across the Baltic Sea countries, with no clear association between genomic repertoire, phylogenomic, or gene distribution and virulence traits. However, analysis of the entire genome of four F. psychrophilum isolates by hybrid assembly pro-vided an unprecedented resolution for discriminating even highly related isolates. The results showed that isolates with different virulence phenotypes harbored genetic variances on a number of consecutive leucine-rich repeat (LRR) proteins, repetitive motifs in gliding motility-associated protein, and the insertion of transposable elements into intergenic and genic regions. Thus, these findings provide novel insights into the genetic variation of these elements and their putative role in the modulation of F. psychrophilum virulence.
AB - The fish pathogen Flavobacterium psychrophilum is currently one of the main pathogenic bacteria hampering the productivity of salmonid farming worldwide. Although putative virulence determinants have been identified, the genetic basis for variation in virulence of F. psychrophilum is not fully understood. In this study, we analyzed whole-genome sequences of a collection of 25 F. psychrophilum isolates from Baltic Sea countries and compared genomic information with a previous determination of their virulence in juvenile rainbow trout. The results revealed a conserved population of F. psychrophilum that were consistently present across the Baltic Sea countries, with no clear association between genomic repertoire, phylogenomic, or gene distribution and virulence traits. However, analysis of the entire genome of four F. psychrophilum isolates by hybrid assembly pro-vided an unprecedented resolution for discriminating even highly related isolates. The results showed that isolates with different virulence phenotypes harbored genetic variances on a number of consecutive leucine-rich repeat (LRR) proteins, repetitive motifs in gliding motility-associated protein, and the insertion of transposable elements into intergenic and genic regions. Thus, these findings provide novel insights into the genetic variation of these elements and their putative role in the modulation of F. psychrophilum virulence.
KW - Evolution
KW - Flavobacterium
KW - Freshwater
KW - Genomics
KW - Pan-genome
KW - Pathogen
KW - Pathogenicity
KW - T9SS
KW - Virulence
U2 - 10.3390/microorganisms9081658
DO - 10.3390/microorganisms9081658
M3 - Journal article
C2 - 34442736
AN - SCOPUS:85111665080
VL - 9
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 8
M1 - 1658
ER -
ID: 279138311