A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples
Research output: Contribution to journal › Journal article › Research › peer-review
We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 μs to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources. A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed.
Original language | English |
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Journal | Sensors and Actuators, B: Chemical |
Volume | 51 |
Issue number | 1-3 |
Pages (from-to) | 163-170 |
Number of pages | 8 |
ISSN | 0925-4005 |
DOIs | |
Publication status | Published - 1998 |
Externally published | Yes |
- Fluorescence lifetime imaging, Luminescence lifetime imaging, Oxygen optode, Planar optode
Research areas
ID: 201682581