Neuropeptide expression in the box jellyfish Tripedalia cystophora - New insights into the complexity of a "simple" nervous system

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Neuropeptide expression in the box jellyfish Tripedalia cystophora - New insights into the complexity of a "simple" nervous system. / Nielsen, Sofie K. D.; Koch, Thomas L.; Wiisbye, Sofus H.; Grimmelikhuijzen, Cornelis J. P.; Garm, Anders.

In: Journal of Comparative Neurology, Vol. 529, No. 11, 2021, p. 2865-2882.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Nielsen, SKD, Koch, TL, Wiisbye, SH, Grimmelikhuijzen, CJP & Garm, A 2021, 'Neuropeptide expression in the box jellyfish Tripedalia cystophora - New insights into the complexity of a "simple" nervous system', Journal of Comparative Neurology, vol. 529, no. 11, pp. 2865-2882. https://doi.org/10.1002/cne.25133

APA

Nielsen, S. K. D., Koch, T. L., Wiisbye, S. H., Grimmelikhuijzen, C. J. P., & Garm, A. (2021). Neuropeptide expression in the box jellyfish Tripedalia cystophora - New insights into the complexity of a "simple" nervous system. Journal of Comparative Neurology, 529(11), 2865-2882. https://doi.org/10.1002/cne.25133

Vancouver

Nielsen SKD, Koch TL, Wiisbye SH, Grimmelikhuijzen CJP, Garm A. Neuropeptide expression in the box jellyfish Tripedalia cystophora - New insights into the complexity of a "simple" nervous system. Journal of Comparative Neurology. 2021;529(11):2865-2882. https://doi.org/10.1002/cne.25133

Author

Nielsen, Sofie K. D. ; Koch, Thomas L. ; Wiisbye, Sofus H. ; Grimmelikhuijzen, Cornelis J. P. ; Garm, Anders. / Neuropeptide expression in the box jellyfish Tripedalia cystophora - New insights into the complexity of a "simple" nervous system. In: Journal of Comparative Neurology. 2021 ; Vol. 529, No. 11. pp. 2865-2882.

Bibtex

@article{0e8d1188d9624d06b258cedefab5b9e2,
title = "Neuropeptide expression in the box jellyfish Tripedalia cystophora - New insights into the complexity of a {"}simple{"} nervous system",
abstract = "Box jellyfish have an elaborate visual system and perform advanced visually guided behaviors. However, the rhopalial nervous system (RNS), believed to be the main visual processing center, only has 1000 neurons in each of the four eye carrying rhopalia. We have examined the detailed structure of the RNS of the box jellyfish Tripedalia cystophora, using immunolabeling with antibodies raised against four putative neuropeptides (T. cystophora RFamide, VWamide, RAamide, and FRamide). In the RNS, T. cystophora RF-, VW-, and RAamide antibodies stain sensory neurons, the pit eyes, the neuropil, and peptide-specific subpopulations of stalk-associated neurons and giant neurons. Furthermore, RFamide ir+ neurites are seen in the epidermal stalk nerve, whereas VWamide antibodies stain the gastrodermal stalk nerve. RFamide has the most widespread expression including in the ring and radial nerves, the pedalium nerve plexus, and the tentacular nerve net. RAamide is the putative neurotransmitter in the motor neurons of the subumbrellar nerve net, and VWamide is a potential marker for neuronal differentiation as it is found in subpopulations of undifferentiated cells both in the rhopalia and in the bell. The results from the FRamide antibodies were not included as only few cells were stained, and in an unreproducible way. Our studies show hitherto-unseen details of the nervous system of T. cystophora and allowed us to identify specific functional groups of neurons. This identification is important for understanding visual processing in the RNS and enables experimental work, directly addressing the role of the different neuropeptides in vision.",
keywords = "central nervous system, Cnidaria, Cubozoa, neuroanatomy, RFamide, visual integration",
author = "Nielsen, {Sofie K. D.} and Koch, {Thomas L.} and Wiisbye, {Sofus H.} and Grimmelikhuijzen, {Cornelis J. P.} and Anders Garm",
year = "2021",
doi = "10.1002/cne.25133",
language = "English",
volume = "529",
pages = "2865--2882",
journal = "The Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "JohnWiley & Sons, Inc.",
number = "11",

}

RIS

TY - JOUR

T1 - Neuropeptide expression in the box jellyfish Tripedalia cystophora - New insights into the complexity of a "simple" nervous system

AU - Nielsen, Sofie K. D.

AU - Koch, Thomas L.

AU - Wiisbye, Sofus H.

AU - Grimmelikhuijzen, Cornelis J. P.

AU - Garm, Anders

PY - 2021

Y1 - 2021

N2 - Box jellyfish have an elaborate visual system and perform advanced visually guided behaviors. However, the rhopalial nervous system (RNS), believed to be the main visual processing center, only has 1000 neurons in each of the four eye carrying rhopalia. We have examined the detailed structure of the RNS of the box jellyfish Tripedalia cystophora, using immunolabeling with antibodies raised against four putative neuropeptides (T. cystophora RFamide, VWamide, RAamide, and FRamide). In the RNS, T. cystophora RF-, VW-, and RAamide antibodies stain sensory neurons, the pit eyes, the neuropil, and peptide-specific subpopulations of stalk-associated neurons and giant neurons. Furthermore, RFamide ir+ neurites are seen in the epidermal stalk nerve, whereas VWamide antibodies stain the gastrodermal stalk nerve. RFamide has the most widespread expression including in the ring and radial nerves, the pedalium nerve plexus, and the tentacular nerve net. RAamide is the putative neurotransmitter in the motor neurons of the subumbrellar nerve net, and VWamide is a potential marker for neuronal differentiation as it is found in subpopulations of undifferentiated cells both in the rhopalia and in the bell. The results from the FRamide antibodies were not included as only few cells were stained, and in an unreproducible way. Our studies show hitherto-unseen details of the nervous system of T. cystophora and allowed us to identify specific functional groups of neurons. This identification is important for understanding visual processing in the RNS and enables experimental work, directly addressing the role of the different neuropeptides in vision.

AB - Box jellyfish have an elaborate visual system and perform advanced visually guided behaviors. However, the rhopalial nervous system (RNS), believed to be the main visual processing center, only has 1000 neurons in each of the four eye carrying rhopalia. We have examined the detailed structure of the RNS of the box jellyfish Tripedalia cystophora, using immunolabeling with antibodies raised against four putative neuropeptides (T. cystophora RFamide, VWamide, RAamide, and FRamide). In the RNS, T. cystophora RF-, VW-, and RAamide antibodies stain sensory neurons, the pit eyes, the neuropil, and peptide-specific subpopulations of stalk-associated neurons and giant neurons. Furthermore, RFamide ir+ neurites are seen in the epidermal stalk nerve, whereas VWamide antibodies stain the gastrodermal stalk nerve. RFamide has the most widespread expression including in the ring and radial nerves, the pedalium nerve plexus, and the tentacular nerve net. RAamide is the putative neurotransmitter in the motor neurons of the subumbrellar nerve net, and VWamide is a potential marker for neuronal differentiation as it is found in subpopulations of undifferentiated cells both in the rhopalia and in the bell. The results from the FRamide antibodies were not included as only few cells were stained, and in an unreproducible way. Our studies show hitherto-unseen details of the nervous system of T. cystophora and allowed us to identify specific functional groups of neurons. This identification is important for understanding visual processing in the RNS and enables experimental work, directly addressing the role of the different neuropeptides in vision.

KW - central nervous system

KW - Cnidaria

KW - Cubozoa

KW - neuroanatomy

KW - RFamide

KW - visual integration

U2 - 10.1002/cne.25133

DO - 10.1002/cne.25133

M3 - Journal article

C2 - 33660861

AN - SCOPUS:85102279674

VL - 529

SP - 2865

EP - 2882

JO - The Journal of Comparative Neurology

JF - The Journal of Comparative Neurology

SN - 0021-9967

IS - 11

ER -

ID: 258767089