A new duck genome reveals conserved and convergently evolved chromosome architectures of birds and mammals

Research output: Contribution to journalJournal articleResearchpeer-review

  • Jing Li
  • Jilin Zhang
  • Jing Liu
  • Yang Zhou
  • Cheng Cai
  • Luohao Xu
  • Xuelei Dai
  • Shaohong Feng
  • Chunxue Guo
  • Jinpeng Rao
  • Kai Wei
  • Erich D. Jarvis
  • Yu Jiang
  • Zhengkui Zhou
  • Zhang, Guojie
  • Qi Zhou

Background: Ducks have a typical avian karyotype that consists of macro-and microchromosomes, but a pair of much less differentiated ZW sex chromosomes compared to chickens. To elucidate the evolution of chromosome architectures between ducks and chickens, and between birds and mammals, we produced a nearly complete chromosomal assembly of a female Pekin duck by combining long-read sequencing and multiplatform scaffolding techniques. Results: A major improvement of genome assembly and annotation quality resulted from the successful resolution of lineage-specific propagated repeats that fragmented the previous Illumina-based assembly. We found that the duck topologically associated domains (TAD) are demarcated by putative binding sites of the insulator protein CTCF, housekeeping genes, or transitions of active/inactive chromatin compartments, indicating conserved mechanisms of spatial chromosome folding with mammals. There are extensive overlaps of TAD boundaries between duck and chicken, and also between the TAD boundaries and chromosome inversion breakpoints. This suggests strong natural selection pressure on maintaining regulatory domain integrity, or vulnerability of TAD boundaries to DNA double-strand breaks. The duck W chromosome retains 2.5-fold more genes relative to chicken. Similar to the independently evolved human Y chromosome, the duck W evolved massive dispersed palindromic structures, and a pattern of sequence divergence with the Z chromosome that reflects stepwise suppression of homologous recombination. Conclusions: Our results provide novel insights into the conserved and convergently evolved chromosome features of birds and mammals, and also importantly add to the genomic resources for poultry studies.

Original languageEnglish
Article numbergiaa142
JournalGigaScience
Volume10
Issue number1
Number of pages15
ISSN2047-217X
DOIs
Publication statusPublished - 2021

    Research areas

  • chromosome inversion, duck genome, sex chromosomes, topologically associated domain

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