A new emu genome illuminates the evolution of genome configuration and nuclear architecture of avian chromosomes

Research output: Contribution to journalJournal articleResearchpeer-review

  • Jing Liu
  • Zongji Wang
  • Jing Li
  • Luohao Xu
  • Jiaqi Liu
  • Shaohong Feng
  • Chunxue Guo
  • Shengchan Chen
  • Zhanjun Ren
  • Jinpeng Rao
  • Kai Wei
  • Yuezhou Chen
  • Erich D. Jarvis
  • Zhang, Guojie
  • Qi Zhou

Emu and other ratites are more informative than any other birds in reconstructing the evolution of the ancestral avian or vertebrate karyotype because of their much slower rate of genome evolution. Here, we generated a new chromosome-level genome assembly of a female emu, and estimated the tempo of chromosome evolution across major avian phylogenetic branches, by comparing it to chromosome-level genome assemblies of 11 other bird and one turtle species. We found ratites exhibited the lowest numbers of intra- and inter-chromosomal changes among birds since their divergence with turtles. The small-sized and gene-rich emu microchromosomes have frequent inter-chromosomal contacts that are associated with housekeeping genes, which appears to be driven by clustering their centromeres in the nuclear interior, away from the macrochromosomes in the nuclear periphery. Unlike nonratite birds, only less than one-third of the emu W Chromosome regions have lost homologous recombination and diverged between the sexes. The emu W is demarcated into a highly heterochromatic region (WS0) and another recently evolved region (WS1) with only moderate sequence divergence with the Z Chromosome. WS1 has expanded its inactive chromatin compartment, increased chromatin contacts within the region, and decreased contacts with the nearby regions, possibly influenced by the spreading of heterochromatin from WS0. These patterns suggest that alteration of chromatin conformation comprises an important early step of sex chromosome evolution. Overall, our results provide novel insights into the evolution of avian genome structure and sex chromosomes in three-dimensional space.

Original languageEnglish
JournalGenome Research
Volume31
Issue number3
Pages (from-to)497-511
Number of pages15
ISSN1088-9051
DOIs
Publication statusPublished - 2021

ID: 260187585