A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues
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- A Sleeping Beauty...
Final published version, 1.47 MB, PDF document
Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that allow a comparative evaluation of drug properties. As a new tool in functional screening of vitamin D3 analogues, we describe a genomically integratable sensor for sensitive drug detection. This system facilitates assessment of the pharmacokinetic and pharmadynamic properties of vitamin D3 analogues. The tri-cistronic genetic sensor encodes a drug-sensoring protein, a reporter protein expressed from an activated sensor-responsive promoter, and a resistance marker.
Original language | English |
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Journal | B M C Biotechnology |
Volume | 11 |
Number of pages | 13 |
ISSN | 1472-6750 |
DOIs | |
Publication status | Published - 2011 |
- Animals, Calcitriol, Cell Line, Cell Proliferation, Cell Survival, Cholecalciferol, DNA Transposable Elements, Dose-Response Relationship, Drug, Drug Evaluation, Preclinical, Genetic Vectors, Green Fluorescent Proteins, Humans, Hydroxycholecalciferols, Keratinocytes, Microscopy, Fluorescence, Mutagenesis, Insertional, Response Elements, Retinoid X Receptors, Transfection, Transposases, Vitamin D-Binding Protein
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ID: 35410253