Ab initio determination of the shape of membrane proteins in a nanodisc

Research output: Contribution to journalJournal articleResearchpeer-review

New software, called Marbles, is introduced that employs SAXS intensities to predict the shape of membrane proteins embedded into membrane nanodiscs. To gain computational speed and efficient convergence, the strategy is based on a hybrid approach that allows one to account for the contribution of the nanodisc to the SAXS intensity through a semi-analytical model, while the embedded membrane protein is treated as a set of beads, similarly to as in well known ab initio methods. The reliability and flexibility of this approach is proved by benchmarking the code, implemented in C++ with a Python interface, on a toy model and two proteins with very different geometry and size.

Original languageEnglish
JournalActa crystallographica Section D: Structural biology
Volume77
Pages (from-to)176-193
Number of pages18
ISSN2059-7983
DOIs
Publication statusPublished - 2021

    Research areas

  • ab initio, membrane proteins, nanodiscs, SAXS, shape

ID: 257540775