African lungfish genome sheds light on the vertebrate water-to-land transition
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
African lungfish genome sheds light on the vertebrate water-to-land transition. / Wang, Kun; Wang, Jun; Zhu, Chenglong; Yang, Liandong; Ren, Yandong; Ruan, Jue; Fan, Guangyi; Hu, Jiang; Xu, Wenjie; Bi, Xupeng; Zhu, Youan; Song, Yue; Chen, Huatao; Ma, Tiantian; Zhao, Ruoping; Jiang, Haifeng; Zhang, Bin; Feng, Chenguang; Yuan, Yuan; Gan, Xiaoni; Li, Yongxin; Zeng, Honghui; Liu, Qun; Zhang, Yaolei; Shao, Feng; Hao, Shijie; Zhang, He; Xu, Xun; Liu, Xin; Wang, Depeng; Zhu, Min; Zhang, Guojie; Zhao, Wenming; Qiu, Qiang; He, Shunping; Wang, Wen.
In: Cell, Vol. 184, No. 5, 2021, p. 1362-1376.e18.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - African lungfish genome sheds light on the vertebrate water-to-land transition
AU - Wang, Kun
AU - Wang, Jun
AU - Zhu, Chenglong
AU - Yang, Liandong
AU - Ren, Yandong
AU - Ruan, Jue
AU - Fan, Guangyi
AU - Hu, Jiang
AU - Xu, Wenjie
AU - Bi, Xupeng
AU - Zhu, Youan
AU - Song, Yue
AU - Chen, Huatao
AU - Ma, Tiantian
AU - Zhao, Ruoping
AU - Jiang, Haifeng
AU - Zhang, Bin
AU - Feng, Chenguang
AU - Yuan, Yuan
AU - Gan, Xiaoni
AU - Li, Yongxin
AU - Zeng, Honghui
AU - Liu, Qun
AU - Zhang, Yaolei
AU - Shao, Feng
AU - Hao, Shijie
AU - Zhang, He
AU - Xu, Xun
AU - Liu, Xin
AU - Wang, Depeng
AU - Zhu, Min
AU - Zhang, Guojie
AU - Zhao, Wenming
AU - Qiu, Qiang
AU - He, Shunping
AU - Wang, Wen
PY - 2021
Y1 - 2021
N2 - Lungfishes are the closest extant relatives of tetrapods and preserve ancestral traits linked with the water-to-land transition. However, their huge genome sizes have hindered understanding of this key transition in evolution. Here, we report a 40-Gb chromosome-level assembly of the African lungfish (Protopterus annectens) genome, which is the largest genome assembly ever reported and has a contig and chromosome N50 of 1.60 Mb and 2.81 Gb, respectively. The large size of the lungfish genome is due mainly to retrotransposons. Genes with ultra-long length show similar expression levels to other genes, indicating that lungfishes have evolved high transcription efficacy to keep gene expression balanced. Together with transcriptome and experimental data, we identified potential genes and regulatory elements related to such terrestrial adaptation traits as pulmonary surfactant, anxiolytic ability, pentadactyl limbs, and pharyngeal remodeling. Our results provide insights and key resources for understanding the evolutionary pathway leading from fishes to humans.
AB - Lungfishes are the closest extant relatives of tetrapods and preserve ancestral traits linked with the water-to-land transition. However, their huge genome sizes have hindered understanding of this key transition in evolution. Here, we report a 40-Gb chromosome-level assembly of the African lungfish (Protopterus annectens) genome, which is the largest genome assembly ever reported and has a contig and chromosome N50 of 1.60 Mb and 2.81 Gb, respectively. The large size of the lungfish genome is due mainly to retrotransposons. Genes with ultra-long length show similar expression levels to other genes, indicating that lungfishes have evolved high transcription efficacy to keep gene expression balanced. Together with transcriptome and experimental data, we identified potential genes and regulatory elements related to such terrestrial adaptation traits as pulmonary surfactant, anxiolytic ability, pentadactyl limbs, and pharyngeal remodeling. Our results provide insights and key resources for understanding the evolutionary pathway leading from fishes to humans.
KW - aestivation
KW - ancestral karyotype
KW - anxiolytic ability
KW - huge genome size
KW - lungfishes
KW - pentadactyl limbs
KW - pharyngeal remodeling
KW - pulmonary surfactant
KW - water-to-land transition
U2 - 10.1016/j.cell.2021.01.047
DO - 10.1016/j.cell.2021.01.047
M3 - Journal article
C2 - 33545087
AN - SCOPUS:85100403772
VL - 184
SP - 1362-1376.e18
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -
ID: 257545099