Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates

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Standard

Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates. / Grimmelikhuijzen, C J; Ebbesen, Ditte Graff.

In: Peptides, Vol. 6 Suppl 3, 01.01.1985, p. 477-83.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Grimmelikhuijzen, CJ & Ebbesen, DG 1985, 'Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates', Peptides, vol. 6 Suppl 3, pp. 477-83.

APA

Grimmelikhuijzen, C. J., & Ebbesen, D. G. (1985). Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates. Peptides, 6 Suppl 3, 477-83.

Vancouver

Grimmelikhuijzen CJ, Ebbesen DG. Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates. Peptides. 1985 Jan 1;6 Suppl 3:477-83.

Author

Grimmelikhuijzen, C J ; Ebbesen, Ditte Graff. / Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates. In: Peptides. 1985 ; Vol. 6 Suppl 3. pp. 477-83.

Bibtex

@article{3ad106953c914d37bf92ec709097f4be,
title = "Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates",
abstract = "By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.",
keywords = "Animals, Chromatography, Cnidaria, FMRFamide, Fluorescent Antibody Technique, Nerve Tissue Proteins, Nervous System Physiological Phenomena, Oligopeptides, Radioimmunoassay",
author = "Grimmelikhuijzen, {C J} and Ebbesen, {Ditte Graff}",
year = "1985",
month = jan,
day = "1",
language = "English",
volume = "6 Suppl 3",
pages = "477--83",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates

AU - Grimmelikhuijzen, C J

AU - Ebbesen, Ditte Graff

PY - 1985/1/1

Y1 - 1985/1/1

N2 - By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.

AB - By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.

KW - Animals

KW - Chromatography

KW - Cnidaria

KW - FMRFamide

KW - Fluorescent Antibody Technique

KW - Nerve Tissue Proteins

KW - Nervous System Physiological Phenomena

KW - Oligopeptides

KW - Radioimmunoassay

M3 - Journal article

C2 - 2870476

VL - 6 Suppl 3

SP - 477

EP - 483

JO - Peptides

JF - Peptides

SN - 0196-9781

ER -

ID: 33514620