Bulk culture levels of specific cytotoxic T-cell activity against HIV-1 proteins are not associated with risk of death
Research output: Contribution to journal › Journal article › Research › peer-review
The ability of cytotoxic T lymphocytes (CTL) to control and influence the outcome of human immunodeficiency virus (HIV) infection is not fully understood. The association between HIV-CTL activity and disease progression was evaluated prospectively in 36 HIV-1-infected individuals with a median follow-up of 3.0 years. HIV-CTL activity was measured in a 4 h Cr* release assay using autologous target cells expressing HIV-1 BRU isolate gene products (gp-120, gag, pol, nef) and a bulk culture of autologous effector cells. The CD4 count was measured at enrolment and plasma HIV RNA was measured retrospectively. The present study failed to support the hypothesis that HIV-CTL activity, as measured using the present method, is important in reducing the risk of death in HIV-infected individuals. However, using other approaches and methods could possibly yield other conclusions, and further prospective studies are needed to examine the relationship between CTL and disease progression.
Original language | English |
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Journal | Scandinavian Journal of Immunology |
Volume | 50 |
Issue number | 2 |
Pages (from-to) | 223-7 |
Number of pages | 5 |
ISSN | 0300-9475 |
Publication status | Published - Aug 1999 |
- Adult, CD4 Lymphocyte Count, Cells, Cultured, Disease Progression, Female, Follow-Up Studies, Gene Products, gag, Gene Products, nef, Gene Products, pol, HIV Antigens, HIV Envelope Protein gp120, HIV Infections, HIV-1, Humans, Male, Middle Aged, RNA, Viral, Retrospective Studies, Risk Factors, T-Lymphocytes, Cytotoxic, nef Gene Products, Human Immunodeficiency Virus, Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 180572145