Distinct localization of FMRFamide- and bovine pancreatic polypeptide-like material in the brain, retrocerebral complex and suboesophageal ganglion of the cockroach Periplaneta americana L
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Distinct localization of FMRFamide- and bovine pancreatic polypeptide-like material in the brain, retrocerebral complex and suboesophageal ganglion of the cockroach Periplaneta americana L. / Verhaert, P; Grimmelikhuijzen, C J; De Loof, A.
In: Brain Research, Vol. 348, No. 2, 02.12.1985, p. 331-8.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Distinct localization of FMRFamide- and bovine pancreatic polypeptide-like material in the brain, retrocerebral complex and suboesophageal ganglion of the cockroach Periplaneta americana L
AU - Verhaert, P
AU - Grimmelikhuijzen, C J
AU - De Loof, A
PY - 1985/12/2
Y1 - 1985/12/2
N2 - One bovine pancreatic polypeptide (BPP) antiserum and two FMRFamide antisera were applied in the peroxidase-antiperoxidase (PAP) immunohistochemical technique on a complete series of sections of brains, suboesophageal ganglia (SOG), corpora cardiaca (CC) and corpora allata of Periplaneta americana L. Double immunohistochemical staining demonstrated that the same perikarya and processes were stained by both the BPP and FMRFamide antisera. This was caused by cross-reaction of the BPP and FMRFamide antisera with common antigenic determinants as was shown by a number of solid-phase absorptions. Application of a third FMRFamide antiserum, which was especially selected for its inability to react with bovine and avian pancreatic polypeptide, showed that more than half of the structures that were stained with the 'unspecific' BPP and FMRFamide antisera, contained material which was genuinely FMRFamide-like. This peptide material was located in cerebral neuronal structures, in the SOG, in the storage site of the CC and in numerous nerve fibres throughout the neuropile regions, which suggested a neurotransmitter/modulator as well as a neurohormonal role. The FMRFamide-like peptide was also found to be present in the same brain sites as an adipokinetic hormone-like peptide, but double labelling revealed that these two substances were never located in the same perikarya or fibres.
AB - One bovine pancreatic polypeptide (BPP) antiserum and two FMRFamide antisera were applied in the peroxidase-antiperoxidase (PAP) immunohistochemical technique on a complete series of sections of brains, suboesophageal ganglia (SOG), corpora cardiaca (CC) and corpora allata of Periplaneta americana L. Double immunohistochemical staining demonstrated that the same perikarya and processes were stained by both the BPP and FMRFamide antisera. This was caused by cross-reaction of the BPP and FMRFamide antisera with common antigenic determinants as was shown by a number of solid-phase absorptions. Application of a third FMRFamide antiserum, which was especially selected for its inability to react with bovine and avian pancreatic polypeptide, showed that more than half of the structures that were stained with the 'unspecific' BPP and FMRFamide antisera, contained material which was genuinely FMRFamide-like. This peptide material was located in cerebral neuronal structures, in the SOG, in the storage site of the CC and in numerous nerve fibres throughout the neuropile regions, which suggested a neurotransmitter/modulator as well as a neurohormonal role. The FMRFamide-like peptide was also found to be present in the same brain sites as an adipokinetic hormone-like peptide, but double labelling revealed that these two substances were never located in the same perikarya or fibres.
KW - Animals
KW - Brain Chemistry
KW - Cattle
KW - Cockroaches
KW - FMRFamide
KW - Ganglia
KW - Immune Sera
KW - Immunoenzyme Techniques
KW - Insect Hormones
KW - Neurons
KW - Oligopeptides
KW - Pancreatic Polypeptide
KW - Staining and Labeling
M3 - Journal article
C2 - 2416381
VL - 348
SP - 331
EP - 338
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -
ID: 33514596