GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B

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Documents

  • Felix Schalk
  • Janis Fricke
  • Soohyun Um
  • Benjamin H. Conlon
  • Hannah Maus
  • Nils Jäger
  • Thorsten Heinzel
  • Tanja Schirmeister
  • Poulsen, Michael
  • Christine Beemelmanns

Targeted HRMS2-GNPS-based metabolomic analysis ofPseudoxylariasp. X187, a fungal antagonist of the fungus-growing termite symbiosis, resulted in the identification of two lipopeptidic congeners of xylacremolides, named xylacremolide C and D, which are built fromd-phenylalanine,l-proline and an acetyl-CoA starter unit elongated by four malonyl-CoA derived ketide units. The putativexyagene cluster was identified from a draft genome generated by Illumina and PacBio sequencing and RNAseq studies. Biological activities of xylacremolide A and B were evaluated and revealed weak histone deacetylase inhibitory (HDACi) and antifungal activities, as well as moderate protease inhibition activity across a panel of nine human, viral and bacterial proteases.

Original languageEnglish
JournalRSC Advances
Volume31
Issue number11
Pages (from-to)18748-18756
Number of pages9
ISSN2046-2069
DOIs
Publication statusPublished - 2021

Bibliographical note

Funding Information:
Funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy - EXC 2051 with Project-ID 390713860 and CRC 1127 with Project-ID 239748522 to CB and TH. Funded by the European Research Council (ERC-CoG - 771349) and The Danish Council for Independent Research (DFF - 7014-00178) to MP. We thank the Humboldt Foundation for a postdoctoral fellowship to SU, and Mrs Heike Heinecke (Hans Kn?ll Institute) for recording NMR spectra.

Funding Information:
Funded by the Deutsche Forschungsgemeinscha (DFG, German Research Foundation) under Germany's Excellence Strategy – EXC 2051 with Project-ID 390713860 and CRC 1127 with Project-ID 239748522 to CB and TH. Funded by the European Research Council (ERC-CoG – 771349) and The Danish Council for Independent Research (DFF – 7014-00178) to MP. We thank the Humboldt Foundation for a postdoctoral fellowship to SU, and Mrs Heike Heinecke (Hans Knöll Institute) for recording NMR spectra.

Publisher Copyright:
© The Royal Society of Chemistry 2021.

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