Seasonal and elevational variability in the induction of specialized compounds from mountain birch (Betula pubescens var. pumila) by winter moth larvae (Operophtera brumata)
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Seasonal and elevational variability in the induction of specialized compounds from mountain birch (Betula pubescens var. pumila) by winter moth larvae (Operophtera brumata). / Ryde, Ingvild; Li, Tao; Rieksta, Jolanta; dos Santos, Bruna Marques; Neilson, Elizabeth H. J.; Gericke, Oliver; Jepsen, Jane U.; Bork, Louise R H; Holm, Hildur S; Rinnan, Riikka.
In: Tree Physiology, Vol. 41, No. 6, 2021, p. 1019-1033.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Seasonal and elevational variability in the induction of specialized compounds from mountain birch (Betula pubescens var. pumila) by winter moth larvae (Operophtera brumata)
AU - Ryde, Ingvild
AU - Li, Tao
AU - Rieksta, Jolanta
AU - dos Santos, Bruna Marques
AU - Neilson, Elizabeth H. J.
AU - Gericke, Oliver
AU - Jepsen, Jane U.
AU - Bork, Louise R H
AU - Holm, Hildur S
AU - Rinnan, Riikka
N1 - CENPERMOA[2021]
PY - 2021
Y1 - 2021
N2 - The mountain birch (Betula pubescens var. pumila (L.)) forest in the Subarctic is periodically exposed to insect outbreaks, which are expected to intensify due to climate change. To mitigate abiotic and biotic stresses, plants have evolved chemical defenses, including volatile organic compounds (VOCs) and non-volatile specialized compounds (NVSCs). Constitutive and induced production of these compounds, however, are poorly studied in Subarctic populations of mountain birch. Here, we assessed the joint effects of insect herbivory, elevation, and season on foliar VOC emissions and NVSC contents of mountain birch. VOCs were sampled in situ by an enclosure technique and analyzed by gas chromatography-mass spectrometry. NVSCs were analyzed by liquid chromatography-mass spectrometry using an untargeted approach. At low elevation, experimental herbivory by winter moth larvae (Operophtera brumata) increased emissions of monoterpenes and homoterpenes over the three-week feeding period, and sesquiterpenes and green leaf volatile in the end of the feeding period. At high elevation, however, herbivory augmented only homoterpene emissions. The more pronounced herbivory effects at low elevation were likely due to higher herbivory intensity. Of the individual compounds, linalool, ocimene, 4,8-dimethylnona-1,3,7-triene, 2-methyl butanenitrile, and benzyl nitrile were among the most responsive compounds in herbivory treatments. Herbivory also altered foliar NVSC profiles at both low and high elevations, with the most responsive compounds likely belonging to fatty acyl glycosides and terpene glycosides. Additionally, VOC emissions from non-infested branches were higher at high than low elevation, particularly during the early season, which was mainly driven by phenological differences. VOC emissions varied substantially over the season, largely reflecting the seasonal variations in temperature and light levels. Our results suggest that if insect herbivory pressure continues to rise in the mountain birch forest with ongoing climate change, it will significantly increase VOC emissions with important consequences for local trophic interactions and climate.
AB - The mountain birch (Betula pubescens var. pumila (L.)) forest in the Subarctic is periodically exposed to insect outbreaks, which are expected to intensify due to climate change. To mitigate abiotic and biotic stresses, plants have evolved chemical defenses, including volatile organic compounds (VOCs) and non-volatile specialized compounds (NVSCs). Constitutive and induced production of these compounds, however, are poorly studied in Subarctic populations of mountain birch. Here, we assessed the joint effects of insect herbivory, elevation, and season on foliar VOC emissions and NVSC contents of mountain birch. VOCs were sampled in situ by an enclosure technique and analyzed by gas chromatography-mass spectrometry. NVSCs were analyzed by liquid chromatography-mass spectrometry using an untargeted approach. At low elevation, experimental herbivory by winter moth larvae (Operophtera brumata) increased emissions of monoterpenes and homoterpenes over the three-week feeding period, and sesquiterpenes and green leaf volatile in the end of the feeding period. At high elevation, however, herbivory augmented only homoterpene emissions. The more pronounced herbivory effects at low elevation were likely due to higher herbivory intensity. Of the individual compounds, linalool, ocimene, 4,8-dimethylnona-1,3,7-triene, 2-methyl butanenitrile, and benzyl nitrile were among the most responsive compounds in herbivory treatments. Herbivory also altered foliar NVSC profiles at both low and high elevations, with the most responsive compounds likely belonging to fatty acyl glycosides and terpene glycosides. Additionally, VOC emissions from non-infested branches were higher at high than low elevation, particularly during the early season, which was mainly driven by phenological differences. VOC emissions varied substantially over the season, largely reflecting the seasonal variations in temperature and light levels. Our results suggest that if insect herbivory pressure continues to rise in the mountain birch forest with ongoing climate change, it will significantly increase VOC emissions with important consequences for local trophic interactions and climate.
U2 - 10.1093/treephys/tpab023
DO - 10.1093/treephys/tpab023
M3 - Journal article
C2 - 33601421
VL - 41
SP - 1019
EP - 1033
JO - Tree Physiology
JF - Tree Physiology
SN - 0829-318X
IS - 6
ER -
ID: 261517429