Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra
Research output: Contribution to journal › Journal article › Research › peer-review
The presence of Arg-Phe-amide (RFamide)-like peptides in dense-cored vesicles in neurons of the peduncle of Hydra was demonstrated by immunogold electron microscopy. Thin sections of Lowicryl-embedded tissue labeled with antisera to RFamide and 5-nm gold-conjugated, secondary antibody and of Epon-Araldite-embedded tissue labeled with 15-nm gold particles revealed a concentration of RFamide-like immunoreactivity over the granular cores of vesicles in epidermal ganglion cells. Gold-labeled, dense-cored vesicles were present in the perikaryon, long thin neurites, and axon terminals of these neurons. The aggregation of labeled dense-cored vesicles in an axon terminal on the myoneme of an epitheliomuscular cell suggests a possible function of RFamide-like peptides in neuromuscular transmission. Gold staining of dense-cored vesicles completely disappeared when the RFamide antiserum was preabsorbed with 10 micrograms/ml RFamide. These results are the first demonstration that the dense-cored vesicles of Hydra neurons contain a neuropeptide.
Original language | English |
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Journal | The Journal of experimental zoology |
Volume | 249 |
Issue number | 1 |
Pages (from-to) | 17-22 |
Number of pages | 6 |
ISSN | 0022-104X |
DOIs | |
Publication status | Published - 1 Jan 1989 |
- Animals, Hydra, Immunohistochemistry, Microscopy, Electron, Neuropeptides
Research areas
ID: 33514299