Beyond Cholera: Characterization of zot-Encoding Filamentous Phages in the Marine Fish Pathogen Vibrio anguillarum
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Beyond Cholera : Characterization of zot-Encoding Filamentous Phages in the Marine Fish Pathogen Vibrio anguillarum. / Mauritzen, Jesper Juel; Castillo, Daniel; Tan, Demeng; Svenningsen, Sine Lo; Middelboe, Mathias.
I: Viruses, Bind 12, Nr. 7, 730, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Beyond Cholera
T2 - Characterization of zot-Encoding Filamentous Phages in the Marine Fish Pathogen Vibrio anguillarum
AU - Mauritzen, Jesper Juel
AU - Castillo, Daniel
AU - Tan, Demeng
AU - Svenningsen, Sine Lo
AU - Middelboe, Mathias
PY - 2020
Y1 - 2020
N2 - Zonula occludens toxin (Zot) is a conserved protein in filamentous vibriophages and has been reported as a putative toxin inVibrio cholerae. Recently, widespread distribution ofzot-encoding prophages was found among marineVibriospecies, including environmental isolates. However, little is known about the dynamics of these prophages beyondV. cholerae. In this study, we characterized and quantified thezot-encoding filamentous phage VAI phi, spontaneously induced from the fish pathogenV. anguillarum. VAI phi contained 6117 bp encoding 11 ORFs, including ORF8(pVAI), exhibiting 27%-73% amino acid identity toInovirusZot-like proteins. A qPCR method revealed an average of four VAI phi genomes per host genome during host exponential growth phase, and PCR demonstrated dissemination of induced VAI phi to otherV. anguillarumstrains through re-integration in non-lysogens. VAI phi integrated into both chromosomes ofV. anguillarumby recombination, causing changes in a putative ORF in the phage genome. Phylogenetic analysis of theV. anguillarumInoviridaeelements revealed mosaic genome structures related to mainlyV. cholerae. Altogether, this study contributes to the understanding ofInovirusinfection dynamics and mobilization ofzot-like genes beyond human pathogenic vibrios, and discusses their potential role in the evolution of the fish pathogenV. anguillarum.
AB - Zonula occludens toxin (Zot) is a conserved protein in filamentous vibriophages and has been reported as a putative toxin inVibrio cholerae. Recently, widespread distribution ofzot-encoding prophages was found among marineVibriospecies, including environmental isolates. However, little is known about the dynamics of these prophages beyondV. cholerae. In this study, we characterized and quantified thezot-encoding filamentous phage VAI phi, spontaneously induced from the fish pathogenV. anguillarum. VAI phi contained 6117 bp encoding 11 ORFs, including ORF8(pVAI), exhibiting 27%-73% amino acid identity toInovirusZot-like proteins. A qPCR method revealed an average of four VAI phi genomes per host genome during host exponential growth phase, and PCR demonstrated dissemination of induced VAI phi to otherV. anguillarumstrains through re-integration in non-lysogens. VAI phi integrated into both chromosomes ofV. anguillarumby recombination, causing changes in a putative ORF in the phage genome. Phylogenetic analysis of theV. anguillarumInoviridaeelements revealed mosaic genome structures related to mainlyV. cholerae. Altogether, this study contributes to the understanding ofInovirusinfection dynamics and mobilization ofzot-like genes beyond human pathogenic vibrios, and discusses their potential role in the evolution of the fish pathogenV. anguillarum.
KW - filamentous phage
KW - Vibrio anguillarum
KW - zot
KW - horizontal gene transfer
KW - marine environment
KW - ZONULA OCCLUDENS TOXIN
KW - VIBRIO-CHOLERAE
KW - GENOMIC SEQUENCE
KW - VGJ-PHI
KW - BACTERIOPHAGE
KW - CTX
KW - INTEGRATION
KW - IDENTIFICATION
KW - RECEPTOR
KW - PREDICTION
U2 - 10.3390/v12070730
DO - 10.3390/v12070730
M3 - Journal article
C2 - 32640584
VL - 12
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 7
M1 - 730
ER -
ID: 246822347