High cell densities favor lysogeny: induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum

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Standard

High cell densities favor lysogeny : induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum. / Tan, Demeng; Hansen, Mads Frederik; de Carvalho, Luís Nunes; Røder, Henriette Lyng; Burmølle, Mette; Middelboe, Mathias; Svenningsen, Sine Lo.

I: The ISME Journal, Bind 14, Nr. 7, 2020, s. 1731-1742.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Tan, D, Hansen, MF, de Carvalho, LN, Røder, HL, Burmølle, M, Middelboe, M & Svenningsen, SL 2020, 'High cell densities favor lysogeny: induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum', The ISME Journal, bind 14, nr. 7, s. 1731-1742. https://doi.org/10.1038/s41396-020-0641-3

APA

Tan, D., Hansen, M. F., de Carvalho, L. N., Røder, H. L., Burmølle, M., Middelboe, M., & Svenningsen, S. L. (2020). High cell densities favor lysogeny: induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum. The ISME Journal, 14(7), 1731-1742. https://doi.org/10.1038/s41396-020-0641-3

Vancouver

Tan D, Hansen MF, de Carvalho LN, Røder HL, Burmølle M, Middelboe M o.a. High cell densities favor lysogeny: induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum. The ISME Journal. 2020;14(7):1731-1742. https://doi.org/10.1038/s41396-020-0641-3

Author

Tan, Demeng ; Hansen, Mads Frederik ; de Carvalho, Luís Nunes ; Røder, Henriette Lyng ; Burmølle, Mette ; Middelboe, Mathias ; Svenningsen, Sine Lo. / High cell densities favor lysogeny : induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum. I: The ISME Journal. 2020 ; Bind 14, Nr. 7. s. 1731-1742.

Bibtex

@article{3a6699170a5e4746ba4f065d2ef3c742,
title = "High cell densities favor lysogeny: induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum",
abstract = "Temperate ϕH20-like phages are repeatedly identified at geographically distinct areas as free phage particles or as prophages of the fish pathogen Vibrio anguillarum. We studied mutants of a lysogenic isolate of V. anguillarum locked in the quorum-sensing regulatory modes of low (ΔvanT) and high (ΔvanO) cell densities by in-frame deletion of key regulators of the quorum-sensing pathway. Remarkably, we find that induction of the H20-like prophage is controlled by the quorum-sensing state of the host, with an eightfold increase in phage particles per cell in high-cell-density cultures of the quorum-sensing-deficient ΔvanT mutant. Comparative studies with prophage-free strains show that biofilm formation is promoted at low cell density and that the H20-like prophage stimulates this behavior. In contrast, the high-cell-density state is associated with reduced prophage induction, increased proteolytic activity, and repression of biofilm. The proteolytic activity may dually function to disperse the biofilm and as a quorum-sensing-mediated antiphage strategy. We demonstrate an intertwined regulation of phage-host interactions and biofilm formation, which is orchestrated by host quorum-sensing signaling, suggesting that increased lysogeny at high cell density is not solely a strategy for phages to piggy-back the successful bacterial hosts but is also a host strategy evolved to take control of the lysis-lysogeny switch to promote host fitness.",
author = "Demeng Tan and Hansen, {Mads Frederik} and {de Carvalho}, {Lu{\'i}s Nunes} and R{\o}der, {Henriette Lyng} and Mette Burm{\o}lle and Mathias Middelboe and Svenningsen, {Sine Lo}",
year = "2020",
doi = "10.1038/s41396-020-0641-3",
language = "English",
volume = "14",
pages = "1731--1742",
journal = "I S M E Journal",
issn = "1751-7362",
publisher = "nature publishing group",
number = "7",

}

RIS

TY - JOUR

T1 - High cell densities favor lysogeny

T2 - induction of an H20 prophage is repressed by quorum sensing and enhances biofilm formation in Vibrio anguillarum

AU - Tan, Demeng

AU - Hansen, Mads Frederik

AU - de Carvalho, Luís Nunes

AU - Røder, Henriette Lyng

AU - Burmølle, Mette

AU - Middelboe, Mathias

AU - Svenningsen, Sine Lo

PY - 2020

Y1 - 2020

N2 - Temperate ϕH20-like phages are repeatedly identified at geographically distinct areas as free phage particles or as prophages of the fish pathogen Vibrio anguillarum. We studied mutants of a lysogenic isolate of V. anguillarum locked in the quorum-sensing regulatory modes of low (ΔvanT) and high (ΔvanO) cell densities by in-frame deletion of key regulators of the quorum-sensing pathway. Remarkably, we find that induction of the H20-like prophage is controlled by the quorum-sensing state of the host, with an eightfold increase in phage particles per cell in high-cell-density cultures of the quorum-sensing-deficient ΔvanT mutant. Comparative studies with prophage-free strains show that biofilm formation is promoted at low cell density and that the H20-like prophage stimulates this behavior. In contrast, the high-cell-density state is associated with reduced prophage induction, increased proteolytic activity, and repression of biofilm. The proteolytic activity may dually function to disperse the biofilm and as a quorum-sensing-mediated antiphage strategy. We demonstrate an intertwined regulation of phage-host interactions and biofilm formation, which is orchestrated by host quorum-sensing signaling, suggesting that increased lysogeny at high cell density is not solely a strategy for phages to piggy-back the successful bacterial hosts but is also a host strategy evolved to take control of the lysis-lysogeny switch to promote host fitness.

AB - Temperate ϕH20-like phages are repeatedly identified at geographically distinct areas as free phage particles or as prophages of the fish pathogen Vibrio anguillarum. We studied mutants of a lysogenic isolate of V. anguillarum locked in the quorum-sensing regulatory modes of low (ΔvanT) and high (ΔvanO) cell densities by in-frame deletion of key regulators of the quorum-sensing pathway. Remarkably, we find that induction of the H20-like prophage is controlled by the quorum-sensing state of the host, with an eightfold increase in phage particles per cell in high-cell-density cultures of the quorum-sensing-deficient ΔvanT mutant. Comparative studies with prophage-free strains show that biofilm formation is promoted at low cell density and that the H20-like prophage stimulates this behavior. In contrast, the high-cell-density state is associated with reduced prophage induction, increased proteolytic activity, and repression of biofilm. The proteolytic activity may dually function to disperse the biofilm and as a quorum-sensing-mediated antiphage strategy. We demonstrate an intertwined regulation of phage-host interactions and biofilm formation, which is orchestrated by host quorum-sensing signaling, suggesting that increased lysogeny at high cell density is not solely a strategy for phages to piggy-back the successful bacterial hosts but is also a host strategy evolved to take control of the lysis-lysogeny switch to promote host fitness.

U2 - 10.1038/s41396-020-0641-3

DO - 10.1038/s41396-020-0641-3

M3 - Journal article

C2 - 32269377

VL - 14

SP - 1731

EP - 1742

JO - I S M E Journal

JF - I S M E Journal

SN - 1751-7362

IS - 7

ER -

ID: 239855690