Lipid Nanodiscs via Ordered Copolymers

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Standard

Lipid Nanodiscs via Ordered Copolymers. / Smith, Anton A. A.; Autzen, Henriette E.; Faust, Bryan; Mann, Joseph L.; Muir, Benjamin W.; Howard, Shaun; Postma, Almar; Spakowitz, Andrew J.; Cheng, Yifan; Appel, Eric A.

I: Chem, Bind 6, Nr. 10, 2020, s. 2782-2795.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Smith, AAA, Autzen, HE, Faust, B, Mann, JL, Muir, BW, Howard, S, Postma, A, Spakowitz, AJ, Cheng, Y & Appel, EA 2020, 'Lipid Nanodiscs via Ordered Copolymers', Chem, bind 6, nr. 10, s. 2782-2795. https://doi.org/10.1016/j.chempr.2020.08.004

APA

Smith, A. A. A., Autzen, H. E., Faust, B., Mann, J. L., Muir, B. W., Howard, S., Postma, A., Spakowitz, A. J., Cheng, Y., & Appel, E. A. (2020). Lipid Nanodiscs via Ordered Copolymers. Chem, 6(10), 2782-2795. https://doi.org/10.1016/j.chempr.2020.08.004

Vancouver

Smith AAA, Autzen HE, Faust B, Mann JL, Muir BW, Howard S o.a. Lipid Nanodiscs via Ordered Copolymers. Chem. 2020;6(10):2782-2795. https://doi.org/10.1016/j.chempr.2020.08.004

Author

Smith, Anton A. A. ; Autzen, Henriette E. ; Faust, Bryan ; Mann, Joseph L. ; Muir, Benjamin W. ; Howard, Shaun ; Postma, Almar ; Spakowitz, Andrew J. ; Cheng, Yifan ; Appel, Eric A. / Lipid Nanodiscs via Ordered Copolymers. I: Chem. 2020 ; Bind 6, Nr. 10. s. 2782-2795.

Bibtex

@article{753f21a802fb4cae82ef57c4c10eada3,
title = "Lipid Nanodiscs via Ordered Copolymers",
abstract = "Amphiphilic copolymers capable of extracting membrane proteins directly from lipid bilayers into “native nanodiscs” promise a simpler membrane protein sample preparation procedure for structural and functional studies. Unfortunately, the selection of nanodisc-forming copolymers is currently limited to molecules that are heterogeneous in terms of molecular weight and monomer sequence, limiting their efficacy in extracting membrane proteins. Here, we report the development of a highly alternating copolymer composed of acrylic acid and styrene by taking advantage of the fundamental reactivity ratios of these monomers. We show that these copolymers, which we term AASTY, are effective solubilizers of membrane proteins expressed in mammalian cells by virtue of their structured amphiphilicity. These AASTY copolymers are promising alternatives to styrene-maleic acid copolymers and provide a new chemical platform for structural and functional characterization of integral membrane proteins in native nanodiscs.",
keywords = "AASTY copolymers, amphiphilic copolymers, lipid nanodiscs, membrane proteins, polymerization, SDG15: Life on land, SDG3: Good health and well-being, single-particle cryo-EM, SMA copolymers",
author = "Smith, {Anton A. A.} and Autzen, {Henriette E.} and Bryan Faust and Mann, {Joseph L.} and Muir, {Benjamin W.} and Shaun Howard and Almar Postma and Spakowitz, {Andrew J.} and Yifan Cheng and Appel, {Eric A.}",
year = "2020",
doi = "10.1016/j.chempr.2020.08.004",
language = "English",
volume = "6",
pages = "2782--2795",
journal = "Chem",
issn = "2451-9294",
publisher = "Elsevier",
number = "10",

}

RIS

TY - JOUR

T1 - Lipid Nanodiscs via Ordered Copolymers

AU - Smith, Anton A. A.

AU - Autzen, Henriette E.

AU - Faust, Bryan

AU - Mann, Joseph L.

AU - Muir, Benjamin W.

AU - Howard, Shaun

AU - Postma, Almar

AU - Spakowitz, Andrew J.

AU - Cheng, Yifan

AU - Appel, Eric A.

PY - 2020

Y1 - 2020

N2 - Amphiphilic copolymers capable of extracting membrane proteins directly from lipid bilayers into “native nanodiscs” promise a simpler membrane protein sample preparation procedure for structural and functional studies. Unfortunately, the selection of nanodisc-forming copolymers is currently limited to molecules that are heterogeneous in terms of molecular weight and monomer sequence, limiting their efficacy in extracting membrane proteins. Here, we report the development of a highly alternating copolymer composed of acrylic acid and styrene by taking advantage of the fundamental reactivity ratios of these monomers. We show that these copolymers, which we term AASTY, are effective solubilizers of membrane proteins expressed in mammalian cells by virtue of their structured amphiphilicity. These AASTY copolymers are promising alternatives to styrene-maleic acid copolymers and provide a new chemical platform for structural and functional characterization of integral membrane proteins in native nanodiscs.

AB - Amphiphilic copolymers capable of extracting membrane proteins directly from lipid bilayers into “native nanodiscs” promise a simpler membrane protein sample preparation procedure for structural and functional studies. Unfortunately, the selection of nanodisc-forming copolymers is currently limited to molecules that are heterogeneous in terms of molecular weight and monomer sequence, limiting their efficacy in extracting membrane proteins. Here, we report the development of a highly alternating copolymer composed of acrylic acid and styrene by taking advantage of the fundamental reactivity ratios of these monomers. We show that these copolymers, which we term AASTY, are effective solubilizers of membrane proteins expressed in mammalian cells by virtue of their structured amphiphilicity. These AASTY copolymers are promising alternatives to styrene-maleic acid copolymers and provide a new chemical platform for structural and functional characterization of integral membrane proteins in native nanodiscs.

KW - AASTY copolymers

KW - amphiphilic copolymers

KW - lipid nanodiscs

KW - membrane proteins

KW - polymerization

KW - SDG15: Life on land

KW - SDG3: Good health and well-being

KW - single-particle cryo-EM

KW - SMA copolymers

U2 - 10.1016/j.chempr.2020.08.004

DO - 10.1016/j.chempr.2020.08.004

M3 - Journal article

AN - SCOPUS:85091235861

VL - 6

SP - 2782

EP - 2795

JO - Chem

JF - Chem

SN - 2451-9294

IS - 10

ER -

ID: 249863221