A porcine brain-wide RNA editing landscape

Research output: Contribution to journalJournal articlepeer-review

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A porcine brain-wide RNA editing landscape. / Huang, Jinrong; Lin, Lin; Dong, Zhanying; Yang, Ling; Zheng, Tianyu; Gu, Weiwang; Zhang, Yan; Yin, Tailang; Sjöstedt, Evelina; Mulder, Jan; Uhlén, Mathias; Kristiansen, Karsten; Bolund, Lars; Luo, Yonglun.

In: Communications Biology , Vol. 4, 717, 2021.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Huang, J, Lin, L, Dong, Z, Yang, L, Zheng, T, Gu, W, Zhang, Y, Yin, T, Sjöstedt, E, Mulder, J, Uhlén, M, Kristiansen, K, Bolund, L & Luo, Y 2021, 'A porcine brain-wide RNA editing landscape', Communications Biology , vol. 4, 717. https://doi.org/10.1038/s42003-021-02238-3

APA

Huang, J., Lin, L., Dong, Z., Yang, L., Zheng, T., Gu, W., Zhang, Y., Yin, T., Sjöstedt, E., Mulder, J., Uhlén, M., Kristiansen, K., Bolund, L., & Luo, Y. (2021). A porcine brain-wide RNA editing landscape. Communications Biology , 4, [717]. https://doi.org/10.1038/s42003-021-02238-3

Vancouver

Huang J, Lin L, Dong Z, Yang L, Zheng T, Gu W et al. A porcine brain-wide RNA editing landscape. Communications Biology . 2021;4. 717. https://doi.org/10.1038/s42003-021-02238-3

Author

Huang, Jinrong ; Lin, Lin ; Dong, Zhanying ; Yang, Ling ; Zheng, Tianyu ; Gu, Weiwang ; Zhang, Yan ; Yin, Tailang ; Sjöstedt, Evelina ; Mulder, Jan ; Uhlén, Mathias ; Kristiansen, Karsten ; Bolund, Lars ; Luo, Yonglun. / A porcine brain-wide RNA editing landscape. In: Communications Biology . 2021 ; Vol. 4.

Bibtex

@article{e1f2b3a56a23454bb730042d9ca439bc,
title = "A porcine brain-wide RNA editing landscape",
abstract = "Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is an essential post-transcriptional modification. Although hundreds of thousands of RNA editing sites have been reported in mammals, brain-wide analysis of the RNA editing in the mammalian brain remains rare. Here, a genome-wide RNA-editing investigation is performed in 119 samples, representing 30 anatomically defined subregions in the pig brain. We identify a total of 682,037 A-to-I RNA editing sites of which 97% are not identified before. Within the pig brain, cerebellum and olfactory bulb are regions with most edited transcripts. The editing level of sites residing in protein-coding regions are similar across brain regions, whereas region-distinct editing is observed in repetitive sequences. Highly edited conserved recoding events in pig and human brain are found in neurotransmitter receptors, demonstrating the evolutionary importance of RNA editing in neurotransmission functions. Although potential data biases caused by age, sex or health status are not considered, this study provides a rich resource to better understand the evolutionary importance of post-transcriptional RNA editing. Huang et al performed a genome-wide RNA editing investigation in the porcine brain in which they found over 680,000 A-to-I RNA editing sites. They identified conserved recoding events between pig and human brains thus providing an extensive resource to aid our understanding of the evolutionary importance of post-transcriptional RNA editing.",
keywords = "ADENOSINE, ALIGNMENT, TRAFFICKING, EXPRESSION, DEFICIENT, DYNAMICS, CHANNELS, TARGETS, BINDING, MOUSE",
author = "Jinrong Huang and Lin Lin and Zhanying Dong and Ling Yang and Tianyu Zheng and Weiwang Gu and Yan Zhang and Tailang Yin and Evelina Sj{\"o}stedt and Jan Mulder and Mathias Uhl{\'e}n and Karsten Kristiansen and Lars Bolund and Yonglun Luo",
year = "2021",
doi = "10.1038/s42003-021-02238-3",
language = "English",
volume = "4",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - A porcine brain-wide RNA editing landscape

AU - Huang, Jinrong

AU - Lin, Lin

AU - Dong, Zhanying

AU - Yang, Ling

AU - Zheng, Tianyu

AU - Gu, Weiwang

AU - Zhang, Yan

AU - Yin, Tailang

AU - Sjöstedt, Evelina

AU - Mulder, Jan

AU - Uhlén, Mathias

AU - Kristiansen, Karsten

AU - Bolund, Lars

AU - Luo, Yonglun

PY - 2021

Y1 - 2021

N2 - Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is an essential post-transcriptional modification. Although hundreds of thousands of RNA editing sites have been reported in mammals, brain-wide analysis of the RNA editing in the mammalian brain remains rare. Here, a genome-wide RNA-editing investigation is performed in 119 samples, representing 30 anatomically defined subregions in the pig brain. We identify a total of 682,037 A-to-I RNA editing sites of which 97% are not identified before. Within the pig brain, cerebellum and olfactory bulb are regions with most edited transcripts. The editing level of sites residing in protein-coding regions are similar across brain regions, whereas region-distinct editing is observed in repetitive sequences. Highly edited conserved recoding events in pig and human brain are found in neurotransmitter receptors, demonstrating the evolutionary importance of RNA editing in neurotransmission functions. Although potential data biases caused by age, sex or health status are not considered, this study provides a rich resource to better understand the evolutionary importance of post-transcriptional RNA editing. Huang et al performed a genome-wide RNA editing investigation in the porcine brain in which they found over 680,000 A-to-I RNA editing sites. They identified conserved recoding events between pig and human brains thus providing an extensive resource to aid our understanding of the evolutionary importance of post-transcriptional RNA editing.

AB - Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is an essential post-transcriptional modification. Although hundreds of thousands of RNA editing sites have been reported in mammals, brain-wide analysis of the RNA editing in the mammalian brain remains rare. Here, a genome-wide RNA-editing investigation is performed in 119 samples, representing 30 anatomically defined subregions in the pig brain. We identify a total of 682,037 A-to-I RNA editing sites of which 97% are not identified before. Within the pig brain, cerebellum and olfactory bulb are regions with most edited transcripts. The editing level of sites residing in protein-coding regions are similar across brain regions, whereas region-distinct editing is observed in repetitive sequences. Highly edited conserved recoding events in pig and human brain are found in neurotransmitter receptors, demonstrating the evolutionary importance of RNA editing in neurotransmission functions. Although potential data biases caused by age, sex or health status are not considered, this study provides a rich resource to better understand the evolutionary importance of post-transcriptional RNA editing. Huang et al performed a genome-wide RNA editing investigation in the porcine brain in which they found over 680,000 A-to-I RNA editing sites. They identified conserved recoding events between pig and human brains thus providing an extensive resource to aid our understanding of the evolutionary importance of post-transcriptional RNA editing.

KW - ADENOSINE

KW - ALIGNMENT

KW - TRAFFICKING

KW - EXPRESSION

KW - DEFICIENT

KW - DYNAMICS

KW - CHANNELS

KW - TARGETS

KW - BINDING

KW - MOUSE

U2 - 10.1038/s42003-021-02238-3

DO - 10.1038/s42003-021-02238-3

M3 - Journal article

C2 - 34112917

VL - 4

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

M1 - 717

ER -

ID: 273369898