A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes

Research output: Contribution to journal › Journal article › Research › peer-review

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A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes. / Kratchmarova, Irina; Kalume, Dario E; Blagoev, Blagoy; Scherer, Philipp E; Podtelejnikov, Alexandre V; Molina, Henrik; Bickel, Perry E; Andersen, Jens S; Fernandez, Minerva M; Bunkenborg, Jacob; Roepstorff, Peter; Kristiansen, Karsten; Lodish, Harvey F; Mann, Matthias; Pandey, Akhilesh.

In: Molecular and Cellular Proteomics, Vol. 1, No. 3, 2002, p. 213-22.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kratchmarova, I, Kalume, DE, Blagoev, B, Scherer, PE, Podtelejnikov, AV, Molina, H, Bickel, PE, Andersen, JS, Fernandez, MM, Bunkenborg, J, Roepstorff, P, Kristiansen, K, Lodish, HF, Mann, M & Pandey, A 2002, 'A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes', Molecular and Cellular Proteomics, vol. 1, no. 3, pp. 213-22.

APA

Kratchmarova, I., Kalume, D. E., Blagoev, B., Scherer, P. E., Podtelejnikov, A. V., Molina, H., Bickel, P. E., Andersen, J. S., Fernandez, M. M., Bunkenborg, J., Roepstorff, P., Kristiansen, K., Lodish, H. F., Mann, M., & Pandey, A. (2002). A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes. Molecular and Cellular Proteomics, 1(3), 213-22.

Vancouver

Kratchmarova I, Kalume DE, Blagoev B, Scherer PE, Podtelejnikov AV, Molina H et al. A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes. Molecular and Cellular Proteomics. 2002;1(3):213-22.

Author

Kratchmarova, Irina ; Kalume, Dario E ; Blagoev, Blagoy ; Scherer, Philipp E ; Podtelejnikov, Alexandre V ; Molina, Henrik ; Bickel, Perry E ; Andersen, Jens S ; Fernandez, Minerva M ; Bunkenborg, Jacob ; Roepstorff, Peter ; Kristiansen, Karsten ; Lodish, Harvey F ; Mann, Matthias ; Pandey, Akhilesh. / A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes. In: Molecular and Cellular Proteomics. 2002 ; Vol. 1, No. 3. pp. 213-22.

Bibtex

@article{88eed8a00e3a11de8478000ea68e967b,
title = "A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes",
abstract = "We have undertaken a systematic proteomic approach to purify and identify secreted factors that are differentially expressed in preadipocytes versus adipocytes. Using one-dimensional gel electrophoresis combined with nanoelectrospray tandem mass spectrometry, proteins that were specifically secreted by 3T3-L1 preadipocytes or adipocytes were identified. In addition to a number of previously reported molecules that are up- or down-regulated during this differentiation process (adipsin, adipocyte complement-related protein 30 kDa, complement C3, and fibronectin), we identified four secreted molecules that have not been shown previously to be expressed differentially during the process of adipogenesis. Pigment epithelium-derived factor, a soluble molecule with potent antiangiogenic properties, was found to be highly secreted by preadipocytes but not adipocytes. Conversely, we found hippocampal cholinergic neurostimulating peptide, neutrophil gelatinase-associated lipocalin, and haptoglobin to be expressed highly by mature adipocytes. We also used liquid chromatography-based separation followed by automated tandem mass spectrometry to identify proteins secreted by mature adipocytes. Several additional secreted proteins including resistin, secreted acidic cysteine-rich glycoprotein/osteonectin, stromal cell-derived factor-1, cystatin C, gelsolin, and matrix metalloprotease-2 were identified by this method. To our knowledge, this is the first study to identify several novel secreted proteins by adipocytes by a proteomic approach using mass spectrometry.",
author = "Irina Kratchmarova and Kalume, {Dario E} and Blagoy Blagoev and Scherer, {Philipp E} and Podtelejnikov, {Alexandre V} and Henrik Molina and Bickel, {Perry E} and Andersen, {Jens S} and Fernandez, {Minerva M} and Jacob Bunkenborg and Peter Roepstorff and Karsten Kristiansen and Lodish, {Harvey F} and Matthias Mann and Akhilesh Pandey",
note = "Keywords: 3T3 Cells; Acute-Phase Proteins; Adipocytes; Animals; Cell Differentiation; Eye Proteins; Gene Expression; Haptoglobins; Lipocalins; Mice; Nerve Growth Factors; Neuropeptides; Oncogene Proteins; Proteins; Proteome; Serpins; Spectrometry, Mass, Electrospray Ionization",
year = "2002",
language = "English",
volume = "1",
pages = "213--22",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology",
number = "3",

}

RIS

TY - JOUR

T1 - A proteomic approach for identification of secreted proteins during the differentiation of 3T3-L1 preadipocytes to adipocytes

AU - Kratchmarova, Irina

AU - Kalume, Dario E

AU - Blagoev, Blagoy

AU - Scherer, Philipp E

AU - Podtelejnikov, Alexandre V

AU - Molina, Henrik

AU - Bickel, Perry E

AU - Andersen, Jens S

AU - Fernandez, Minerva M

AU - Bunkenborg, Jacob

AU - Roepstorff, Peter

AU - Kristiansen, Karsten

AU - Lodish, Harvey F

AU - Mann, Matthias

AU - Pandey, Akhilesh

N1 - Keywords: 3T3 Cells; Acute-Phase Proteins; Adipocytes; Animals; Cell Differentiation; Eye Proteins; Gene Expression; Haptoglobins; Lipocalins; Mice; Nerve Growth Factors; Neuropeptides; Oncogene Proteins; Proteins; Proteome; Serpins; Spectrometry, Mass, Electrospray Ionization

PY - 2002

Y1 - 2002

N2 - We have undertaken a systematic proteomic approach to purify and identify secreted factors that are differentially expressed in preadipocytes versus adipocytes. Using one-dimensional gel electrophoresis combined with nanoelectrospray tandem mass spectrometry, proteins that were specifically secreted by 3T3-L1 preadipocytes or adipocytes were identified. In addition to a number of previously reported molecules that are up- or down-regulated during this differentiation process (adipsin, adipocyte complement-related protein 30 kDa, complement C3, and fibronectin), we identified four secreted molecules that have not been shown previously to be expressed differentially during the process of adipogenesis. Pigment epithelium-derived factor, a soluble molecule with potent antiangiogenic properties, was found to be highly secreted by preadipocytes but not adipocytes. Conversely, we found hippocampal cholinergic neurostimulating peptide, neutrophil gelatinase-associated lipocalin, and haptoglobin to be expressed highly by mature adipocytes. We also used liquid chromatography-based separation followed by automated tandem mass spectrometry to identify proteins secreted by mature adipocytes. Several additional secreted proteins including resistin, secreted acidic cysteine-rich glycoprotein/osteonectin, stromal cell-derived factor-1, cystatin C, gelsolin, and matrix metalloprotease-2 were identified by this method. To our knowledge, this is the first study to identify several novel secreted proteins by adipocytes by a proteomic approach using mass spectrometry.

AB - We have undertaken a systematic proteomic approach to purify and identify secreted factors that are differentially expressed in preadipocytes versus adipocytes. Using one-dimensional gel electrophoresis combined with nanoelectrospray tandem mass spectrometry, proteins that were specifically secreted by 3T3-L1 preadipocytes or adipocytes were identified. In addition to a number of previously reported molecules that are up- or down-regulated during this differentiation process (adipsin, adipocyte complement-related protein 30 kDa, complement C3, and fibronectin), we identified four secreted molecules that have not been shown previously to be expressed differentially during the process of adipogenesis. Pigment epithelium-derived factor, a soluble molecule with potent antiangiogenic properties, was found to be highly secreted by preadipocytes but not adipocytes. Conversely, we found hippocampal cholinergic neurostimulating peptide, neutrophil gelatinase-associated lipocalin, and haptoglobin to be expressed highly by mature adipocytes. We also used liquid chromatography-based separation followed by automated tandem mass spectrometry to identify proteins secreted by mature adipocytes. Several additional secreted proteins including resistin, secreted acidic cysteine-rich glycoprotein/osteonectin, stromal cell-derived factor-1, cystatin C, gelsolin, and matrix metalloprotease-2 were identified by this method. To our knowledge, this is the first study to identify several novel secreted proteins by adipocytes by a proteomic approach using mass spectrometry.

M3 - Journal article

C2 - 12096121

VL - 1

SP - 213

EP - 222

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

SN - 1535-9476

IS - 3

ER -

ID: 11206399