An improved PCR-based method for site directed mutagenesis using megaprimers

Research output: Contribution to journalJournal articlepeer-review

Standard

An improved PCR-based method for site directed mutagenesis using megaprimers. / Brøns-Poulsen, J; Petersen, N E; Hørder, M; Kristiansen, K.

In: Molecular and Cellular Probes, Vol. 12, No. 6, 1998, p. 345-8.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Brøns-Poulsen, J, Petersen, NE, Hørder, M & Kristiansen, K 1998, 'An improved PCR-based method for site directed mutagenesis using megaprimers', Molecular and Cellular Probes, vol. 12, no. 6, pp. 345-8. https://doi.org/10.1006/mcpr.1998.0187

APA

Brøns-Poulsen, J., Petersen, N. E., Hørder, M., & Kristiansen, K. (1998). An improved PCR-based method for site directed mutagenesis using megaprimers. Molecular and Cellular Probes, 12(6), 345-8. https://doi.org/10.1006/mcpr.1998.0187

Vancouver

Brøns-Poulsen J, Petersen NE, Hørder M, Kristiansen K. An improved PCR-based method for site directed mutagenesis using megaprimers. Molecular and Cellular Probes. 1998;12(6):345-8. https://doi.org/10.1006/mcpr.1998.0187

Author

Brøns-Poulsen, J ; Petersen, N E ; Hørder, M ; Kristiansen, K. / An improved PCR-based method for site directed mutagenesis using megaprimers. In: Molecular and Cellular Probes. 1998 ; Vol. 12, No. 6. pp. 345-8.

Bibtex

@article{b75a3ef00fac11de8478000ea68e967b,
title = "An improved PCR-based method for site directed mutagenesis using megaprimers",
abstract = "An improved protocol for site-directed mutagenesis based on the two-step polymerase chain reaction (PCR) megaprimer method is described. Compared to previously published protocols, the protocol described in this article ensures consistently a success rate of at least 85% with essentially no introduction of unwanted secondary mutations. The essential features of this protocol include an optimization of the template-primer amounts and ratio that allows the use of a reduced number of PCR cycles and the use of proof-reading thermostable DNA polymerases.",
author = "J Br{\o}ns-Poulsen and Petersen, {N E} and M H{\o}rder and K Kristiansen",
note = "Keywords: DNA Primers; DNA-Directed DNA Polymerase; Humans; Hydroxymethylbilane Synthase; Mutagenesis, Site-Directed; Point Mutation; Polymerase Chain Reaction",
year = "1998",
doi = "10.1006/mcpr.1998.0187",
language = "English",
volume = "12",
pages = "345--8",
journal = "Molecular and Cellular Probes",
issn = "0890-8508",
publisher = "Academic Press",
number = "6",

}

RIS

TY - JOUR

T1 - An improved PCR-based method for site directed mutagenesis using megaprimers

AU - Brøns-Poulsen, J

AU - Petersen, N E

AU - Hørder, M

AU - Kristiansen, K

N1 - Keywords: DNA Primers; DNA-Directed DNA Polymerase; Humans; Hydroxymethylbilane Synthase; Mutagenesis, Site-Directed; Point Mutation; Polymerase Chain Reaction

PY - 1998

Y1 - 1998

N2 - An improved protocol for site-directed mutagenesis based on the two-step polymerase chain reaction (PCR) megaprimer method is described. Compared to previously published protocols, the protocol described in this article ensures consistently a success rate of at least 85% with essentially no introduction of unwanted secondary mutations. The essential features of this protocol include an optimization of the template-primer amounts and ratio that allows the use of a reduced number of PCR cycles and the use of proof-reading thermostable DNA polymerases.

AB - An improved protocol for site-directed mutagenesis based on the two-step polymerase chain reaction (PCR) megaprimer method is described. Compared to previously published protocols, the protocol described in this article ensures consistently a success rate of at least 85% with essentially no introduction of unwanted secondary mutations. The essential features of this protocol include an optimization of the template-primer amounts and ratio that allows the use of a reduced number of PCR cycles and the use of proof-reading thermostable DNA polymerases.

U2 - 10.1006/mcpr.1998.0187

DO - 10.1006/mcpr.1998.0187

M3 - Journal article

C2 - 9843651

VL - 12

SP - 345

EP - 348

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

SN - 0890-8508

IS - 6

ER -

ID: 11254093