An intron in a ribosomal protein gene from Tetrahymena

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An intron in a ribosomal protein gene from Tetrahymena. / Nielsen, Henrik; Andreasen, Per Hove; Dreisig, Hanne; Kristiansen, Karsten; Engberg, Jan.

In: EMBO Journal, Vol. 5, No. 10, 1986, p. 2711-2717.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Nielsen, H, Andreasen, PH, Dreisig, H, Kristiansen, K & Engberg, J 1986, 'An intron in a ribosomal protein gene from Tetrahymena', EMBO Journal, vol. 5, no. 10, pp. 2711-2717. <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1167173>

APA

Nielsen, H., Andreasen, P. H., Dreisig, H., Kristiansen, K., & Engberg, J. (1986). An intron in a ribosomal protein gene from Tetrahymena. EMBO Journal, 5(10), 2711-2717. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1167173

Vancouver

Nielsen H, Andreasen PH, Dreisig H, Kristiansen K, Engberg J. An intron in a ribosomal protein gene from Tetrahymena. EMBO Journal. 1986;5(10):2711-2717.

Author

Nielsen, Henrik ; Andreasen, Per Hove ; Dreisig, Hanne ; Kristiansen, Karsten ; Engberg, Jan. / An intron in a ribosomal protein gene from Tetrahymena. In: EMBO Journal. 1986 ; Vol. 5, No. 10. pp. 2711-2717.

Bibtex

@article{2f79576013c511de8478000ea68e967b,
title = "An intron in a ribosomal protein gene from Tetrahymena",
abstract = "We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.",
author = "Henrik Nielsen and Andreasen, {Per Hove} and Hanne Dreisig and Karsten Kristiansen and Jan Engberg",
year = "1986",
language = "English",
volume = "5",
pages = "2711--2717",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "10",

}

RIS

TY - JOUR

T1 - An intron in a ribosomal protein gene from Tetrahymena

AU - Nielsen, Henrik

AU - Andreasen, Per Hove

AU - Dreisig, Hanne

AU - Kristiansen, Karsten

AU - Engberg, Jan

PY - 1986

Y1 - 1986

N2 - We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.

AB - We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.

M3 - Journal article

C2 - 16453715

VL - 5

SP - 2711

EP - 2717

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 10

ER -

ID: 11368404