An intron in a ribosomal protein gene from Tetrahymena
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An intron in a ribosomal protein gene from Tetrahymena. / Nielsen, Henrik; Andreasen, Per Hove; Dreisig, Hanne; Kristiansen, Karsten; Engberg, Jan.
In: EMBO Journal, Vol. 5, No. 10, 1986, p. 2711-2717.Research output: Contribution to journal › Journal article › peer-review
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T1 - An intron in a ribosomal protein gene from Tetrahymena
AU - Nielsen, Henrik
AU - Andreasen, Per Hove
AU - Dreisig, Hanne
AU - Kristiansen, Karsten
AU - Engberg, Jan
PY - 1986
Y1 - 1986
N2 - We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.
AB - We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.
M3 - Journal article
C2 - 16453715
VL - 5
SP - 2711
EP - 2717
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 10
ER -
ID: 11368404