Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes

Research output: Contribution to journalJournal articlepeer-review

Standard

Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes. / Pinilla-Redondo, Rafael; Olesen, Asmus Kalckar; Russel, Jakob; de Vries, Lisbeth Elvira; Christensen, Lisbeth Damkjær; Musovic, Sanin; Nesme, Joseph; Sørensen, Søren Johannes.

In: mBio, Vol. 12, No. 6, e0306821, 2021.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Pinilla-Redondo, R, Olesen, AK, Russel, J, de Vries, LE, Christensen, LD, Musovic, S, Nesme, J & Sørensen, SJ 2021, 'Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes', mBio, vol. 12, no. 6, e0306821. https://doi.org/10.1128/mBio.03068-21

APA

Pinilla-Redondo, R., Olesen, A. K., Russel, J., de Vries, L. E., Christensen, L. D., Musovic, S., Nesme, J., & Sørensen, S. J. (2021). Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes. mBio, 12(6), [e0306821]. https://doi.org/10.1128/mBio.03068-21

Vancouver

Pinilla-Redondo R, Olesen AK, Russel J, de Vries LE, Christensen LD, Musovic S et al. Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes. mBio. 2021;12(6). e0306821. https://doi.org/10.1128/mBio.03068-21

Author

Pinilla-Redondo, Rafael ; Olesen, Asmus Kalckar ; Russel, Jakob ; de Vries, Lisbeth Elvira ; Christensen, Lisbeth Damkjær ; Musovic, Sanin ; Nesme, Joseph ; Sørensen, Søren Johannes. / Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes. In: mBio. 2021 ; Vol. 12, No. 6.

Bibtex

@article{aa2e17fbe4794364949f2ce65b1e98b6,
title = "Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes",
abstract = "Biological rapid sand filtration is a commonly employed method for the removal of organic and inorganic impurities in water which relies on the degradative properties of microorganisms for the removal of diverse contaminants, but their bioremediation capabilities vary greatly across waterworks. Bioaugmentation efforts with degradation-proficient bacteria have proven difficult due to the inability of the exogenous microbes to stably colonize the sand filters. Plasmids are extrachromosomal DNA elements that can often transfer between bacteria and facilitate the flow of genetic information across microbiomes, yet their ability to spread within rapid sand filters has remained unknown. Here, we examine the permissiveness of rapid sand filter communities toward four environmentally transmissible plasmids, RP4, RSF1010, pKJK5, and TOL (pWWO), using a dual-fluorescence bioreporter platform combined with fluorescence-activated cell sorting (FACS) and 16S rRNA gene amplicon sequencing. Our results reveal that plasmids can transfer at high frequencies and across distantly related taxa from rapid sand filter communities, emphasizing their potential suitability for introducing bioremediation determinants in the microbiomes of underperforming water purification plants.",
keywords = "Conjugation, Horizontal gene transfer, Microbial communities, Mobile genetic elements, Plasmid, Plasmid dissemination, Plasmid host range, Plasmid transfer, Rapid sand filters",
author = "Rafael Pinilla-Redondo and Olesen, {Asmus Kalckar} and Jakob Russel and {de Vries}, {Lisbeth Elvira} and Christensen, {Lisbeth Damkj{\ae}r} and Sanin Musovic and Joseph Nesme and S{\o}rensen, {S{\o}ren Johannes}",
note = "Publisher Copyright: Copyright {\textcopyright} 2021 Pinilla-Redondo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.",
year = "2021",
doi = "10.1128/mBio.03068-21",
language = "English",
volume = "12",
journal = "mBio",
issn = "2161-2129",
publisher = "American Society for Microbiology",
number = "6",

}

RIS

TY - JOUR

T1 - Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes

AU - Pinilla-Redondo, Rafael

AU - Olesen, Asmus Kalckar

AU - Russel, Jakob

AU - de Vries, Lisbeth Elvira

AU - Christensen, Lisbeth Damkjær

AU - Musovic, Sanin

AU - Nesme, Joseph

AU - Sørensen, Søren Johannes

N1 - Publisher Copyright: Copyright © 2021 Pinilla-Redondo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PY - 2021

Y1 - 2021

N2 - Biological rapid sand filtration is a commonly employed method for the removal of organic and inorganic impurities in water which relies on the degradative properties of microorganisms for the removal of diverse contaminants, but their bioremediation capabilities vary greatly across waterworks. Bioaugmentation efforts with degradation-proficient bacteria have proven difficult due to the inability of the exogenous microbes to stably colonize the sand filters. Plasmids are extrachromosomal DNA elements that can often transfer between bacteria and facilitate the flow of genetic information across microbiomes, yet their ability to spread within rapid sand filters has remained unknown. Here, we examine the permissiveness of rapid sand filter communities toward four environmentally transmissible plasmids, RP4, RSF1010, pKJK5, and TOL (pWWO), using a dual-fluorescence bioreporter platform combined with fluorescence-activated cell sorting (FACS) and 16S rRNA gene amplicon sequencing. Our results reveal that plasmids can transfer at high frequencies and across distantly related taxa from rapid sand filter communities, emphasizing their potential suitability for introducing bioremediation determinants in the microbiomes of underperforming water purification plants.

AB - Biological rapid sand filtration is a commonly employed method for the removal of organic and inorganic impurities in water which relies on the degradative properties of microorganisms for the removal of diverse contaminants, but their bioremediation capabilities vary greatly across waterworks. Bioaugmentation efforts with degradation-proficient bacteria have proven difficult due to the inability of the exogenous microbes to stably colonize the sand filters. Plasmids are extrachromosomal DNA elements that can often transfer between bacteria and facilitate the flow of genetic information across microbiomes, yet their ability to spread within rapid sand filters has remained unknown. Here, we examine the permissiveness of rapid sand filter communities toward four environmentally transmissible plasmids, RP4, RSF1010, pKJK5, and TOL (pWWO), using a dual-fluorescence bioreporter platform combined with fluorescence-activated cell sorting (FACS) and 16S rRNA gene amplicon sequencing. Our results reveal that plasmids can transfer at high frequencies and across distantly related taxa from rapid sand filter communities, emphasizing their potential suitability for introducing bioremediation determinants in the microbiomes of underperforming water purification plants.

KW - Conjugation

KW - Horizontal gene transfer

KW - Microbial communities

KW - Mobile genetic elements

KW - Plasmid

KW - Plasmid dissemination

KW - Plasmid host range

KW - Plasmid transfer

KW - Rapid sand filters

U2 - 10.1128/mBio.03068-21

DO - 10.1128/mBio.03068-21

M3 - Journal article

C2 - 34844427

AN - SCOPUS:85121973523

VL - 12

JO - mBio

JF - mBio

SN - 2161-2129

IS - 6

M1 - e0306821

ER -

ID: 291214738