Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes
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Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes. / Pinilla-Redondo, Rafael; Olesen, Asmus Kalckar; Russel, Jakob; de Vries, Lisbeth Elvira; Christensen, Lisbeth Damkjær; Musovic, Sanin; Nesme, Joseph; Sørensen, Søren Johannes.
In: mBio, Vol. 12, No. 6, e0306821, 2021.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Broad Dissemination of Plasmids across Groundwater-Fed Rapid Sand Filter Microbiomes
AU - Pinilla-Redondo, Rafael
AU - Olesen, Asmus Kalckar
AU - Russel, Jakob
AU - de Vries, Lisbeth Elvira
AU - Christensen, Lisbeth Damkjær
AU - Musovic, Sanin
AU - Nesme, Joseph
AU - Sørensen, Søren Johannes
N1 - Publisher Copyright: Copyright © 2021 Pinilla-Redondo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
PY - 2021
Y1 - 2021
N2 - Biological rapid sand filtration is a commonly employed method for the removal of organic and inorganic impurities in water which relies on the degradative properties of microorganisms for the removal of diverse contaminants, but their bioremediation capabilities vary greatly across waterworks. Bioaugmentation efforts with degradation-proficient bacteria have proven difficult due to the inability of the exogenous microbes to stably colonize the sand filters. Plasmids are extrachromosomal DNA elements that can often transfer between bacteria and facilitate the flow of genetic information across microbiomes, yet their ability to spread within rapid sand filters has remained unknown. Here, we examine the permissiveness of rapid sand filter communities toward four environmentally transmissible plasmids, RP4, RSF1010, pKJK5, and TOL (pWWO), using a dual-fluorescence bioreporter platform combined with fluorescence-activated cell sorting (FACS) and 16S rRNA gene amplicon sequencing. Our results reveal that plasmids can transfer at high frequencies and across distantly related taxa from rapid sand filter communities, emphasizing their potential suitability for introducing bioremediation determinants in the microbiomes of underperforming water purification plants.
AB - Biological rapid sand filtration is a commonly employed method for the removal of organic and inorganic impurities in water which relies on the degradative properties of microorganisms for the removal of diverse contaminants, but their bioremediation capabilities vary greatly across waterworks. Bioaugmentation efforts with degradation-proficient bacteria have proven difficult due to the inability of the exogenous microbes to stably colonize the sand filters. Plasmids are extrachromosomal DNA elements that can often transfer between bacteria and facilitate the flow of genetic information across microbiomes, yet their ability to spread within rapid sand filters has remained unknown. Here, we examine the permissiveness of rapid sand filter communities toward four environmentally transmissible plasmids, RP4, RSF1010, pKJK5, and TOL (pWWO), using a dual-fluorescence bioreporter platform combined with fluorescence-activated cell sorting (FACS) and 16S rRNA gene amplicon sequencing. Our results reveal that plasmids can transfer at high frequencies and across distantly related taxa from rapid sand filter communities, emphasizing their potential suitability for introducing bioremediation determinants in the microbiomes of underperforming water purification plants.
KW - Conjugation
KW - Horizontal gene transfer
KW - Microbial communities
KW - Mobile genetic elements
KW - Plasmid
KW - Plasmid dissemination
KW - Plasmid host range
KW - Plasmid transfer
KW - Rapid sand filters
U2 - 10.1128/mBio.03068-21
DO - 10.1128/mBio.03068-21
M3 - Journal article
C2 - 34844427
AN - SCOPUS:85121973523
VL - 12
JO - mBio
JF - mBio
SN - 2161-2129
IS - 6
M1 - e0306821
ER -
ID: 291214738