Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces
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Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces. / Zou, Yuanqiang; Lin, Xiaoqian; Xue, Wenbin; Tuo, Li; Chen, Ming-Sheng; Chen, Xiao-Hui; Sun, Cheng-hang; Li, Feina; Liu, Shao-wei; Dai, Ying; Kristiansen, Karsten; Xiao, Liang.
In: Scientific Reports, Vol. 11, 11340, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces
AU - Zou, Yuanqiang
AU - Lin, Xiaoqian
AU - Xue, Wenbin
AU - Tuo, Li
AU - Chen, Ming-Sheng
AU - Chen, Xiao-Hui
AU - Sun, Cheng-hang
AU - Li, Feina
AU - Liu, Shao-wei
AU - Dai, Ying
AU - Kristiansen, Karsten
AU - Xiao, Liang
PY - 2021
Y1 - 2021
N2 - Exploiting a pure culture strategy to investigate the composition of the human gut microbiota, two novel anaerobes, designated strains AF52-21(T) and CM04-06(T), were isolated from faeces of two healthy Chinese donors and characterized using a polyphasic approach. The two strains were observed to be gram-negative, non-motile, and rod-shaped. Both strains grew optimally at 37 degrees C and pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains clustered with species of the genus Faecalibacterium and were most closely related to Faecalibacterium prausnitzii ATCC 27768(T) with sequence similarity of 97.18% and 96.87%, respectively. The two isolates shared a 16S rRNA gene sequence identity of 98.69%. Draft genome sequencing was performed for strains AF52-21(T) and CM04-06(T), generating genome sizes of 2.85 Mbp and 3.01 Mbp. The calculated average nucleotide identity values between the genomes of the strains AF52-21(T) and CM04-06(T) compared to Faecalibacterium prausnitzii ATCC 27768(T) were 83.20% and 82.54%, respectively, and 90.09% when comparing AF52-21(T) and CM04-06(T). Both values were below the previously proposed species threshold (95-96%), supporting their recognition as novel species in the genus Faecalibacterium. The genomic DNA G+C contents of strains AF52-21(T) and CM04-06(T) calculated from genome sequences were 57.77 mol% and 57.51 mol%, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic characteristics, we conclude that both strains represent two new Faecalibacterium species, for which the names Faecalibacterium butyricigenerans sp. nov. (type strain AF52-21(T)=CGMCC 1.5206(T)=DSM 103434(T)) and Faecalibacterium longum sp. nov. (type strain CM04-06(T)=CGMCC 1.5208(T)=DSM 103432(T)) are proposed.
AB - Exploiting a pure culture strategy to investigate the composition of the human gut microbiota, two novel anaerobes, designated strains AF52-21(T) and CM04-06(T), were isolated from faeces of two healthy Chinese donors and characterized using a polyphasic approach. The two strains were observed to be gram-negative, non-motile, and rod-shaped. Both strains grew optimally at 37 degrees C and pH 7.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains clustered with species of the genus Faecalibacterium and were most closely related to Faecalibacterium prausnitzii ATCC 27768(T) with sequence similarity of 97.18% and 96.87%, respectively. The two isolates shared a 16S rRNA gene sequence identity of 98.69%. Draft genome sequencing was performed for strains AF52-21(T) and CM04-06(T), generating genome sizes of 2.85 Mbp and 3.01 Mbp. The calculated average nucleotide identity values between the genomes of the strains AF52-21(T) and CM04-06(T) compared to Faecalibacterium prausnitzii ATCC 27768(T) were 83.20% and 82.54%, respectively, and 90.09% when comparing AF52-21(T) and CM04-06(T). Both values were below the previously proposed species threshold (95-96%), supporting their recognition as novel species in the genus Faecalibacterium. The genomic DNA G+C contents of strains AF52-21(T) and CM04-06(T) calculated from genome sequences were 57.77 mol% and 57.51 mol%, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic characteristics, we conclude that both strains represent two new Faecalibacterium species, for which the names Faecalibacterium butyricigenerans sp. nov. (type strain AF52-21(T)=CGMCC 1.5206(T)=DSM 103434(T)) and Faecalibacterium longum sp. nov. (type strain CM04-06(T)=CGMCC 1.5208(T)=DSM 103432(T)) are proposed.
KW - GUT MICROBIOTA COMPOSITION
KW - GENE SEQUENCE SIMILARITY
KW - FUSOBACTERIUM-PRAUSNITZII
KW - GENOMES
KW - DISEASE
KW - ABUNDANCE
KW - OBESITY
KW - VALUES
KW - SERVER
KW - DIET
U2 - 10.1038/s41598-021-90786-3
DO - 10.1038/s41598-021-90786-3
M3 - Journal article
C2 - 34059761
VL - 11
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 11340
ER -
ID: 273371433