Chitin and stress induced protein kinase activation

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.

Original languageEnglish
Title of host publicationPlant pattern recognition receptors : methods and protocols
EditorsLibo Shan, Ping He
Number of pages10
PublisherSpringer
Publication date2017
Pages185-194
Chapter15
ISBN (Print)978-1-4939-6858-9
ISBN (Electronic)978-1-4939-6859-6
DOIs
Publication statusPublished - 2017
SeriesMethods in Molecular Biology
Volume1578
ISSN1064-3745

    Research areas

  • Acryloylated peptide substrates, In-gel assays, Myelin basic protein

ID: 181454851