Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis
Research output: Contribution to journal › Journal article › Research › peer-review
The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.
|Journal||European Journal of Biochemistry|
|Number of pages||5|
|Publication status||Published - 1978|
Keywords: Acetone; Animals; Cell Fractionation; Molecular Weight; Protein Biosynthesis; Ribosomal Proteins; Ribosomes; Tetrahymena pyriformis