Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis

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The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.
Original languageEnglish
JournalEuropean Journal of Biochemistry
Issue number2
Pages (from-to)389-94
Number of pages5
Publication statusPublished - 1978
Externally publishedYes

Bibliographical note

Keywords: Acetone; Animals; Cell Fractionation; Molecular Weight; Protein Biosynthesis; Ribosomal Proteins; Ribosomes; Tetrahymena pyriformis

ID: 11368600