CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids

Research output: Contribution to journalJournal articlepeer-review

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CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids. / Pinilla-Redondo, Rafael; Russel, Jakob; Mayo-Muñoz, David; Shah, Shiraz A.; Garrett, Roger A.; Nesme, Joseph; Madsen, Jonas S.; Fineran, Peter C.; Sørensen, Søren J.

In: Nucleic Acids Research, Vol. 50, No. 8, 2022, p. 4315-4328.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Pinilla-Redondo, R, Russel, J, Mayo-Muñoz, D, Shah, SA, Garrett, RA, Nesme, J, Madsen, JS, Fineran, PC & Sørensen, SJ 2022, 'CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids', Nucleic Acids Research, vol. 50, no. 8, pp. 4315-4328. https://doi.org/10.1093/nar/gkab859

APA

Pinilla-Redondo, R., Russel, J., Mayo-Muñoz, D., Shah, S. A., Garrett, R. A., Nesme, J., Madsen, J. S., Fineran, P. C., & Sørensen, S. J. (2022). CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids. Nucleic Acids Research, 50(8), 4315-4328. https://doi.org/10.1093/nar/gkab859

Vancouver

Pinilla-Redondo R, Russel J, Mayo-Muñoz D, Shah SA, Garrett RA, Nesme J et al. CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids. Nucleic Acids Research. 2022;50(8):4315-4328. https://doi.org/10.1093/nar/gkab859

Author

Pinilla-Redondo, Rafael ; Russel, Jakob ; Mayo-Muñoz, David ; Shah, Shiraz A. ; Garrett, Roger A. ; Nesme, Joseph ; Madsen, Jonas S. ; Fineran, Peter C. ; Sørensen, Søren J. / CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids. In: Nucleic Acids Research. 2022 ; Vol. 50, No. 8. pp. 4315-4328.

Bibtex

@article{61f612fbf72c49e5aea7196ca61846e2,
title = "CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids",
abstract = "Many prokaryotes encode CRISPR-Cas systems as immune protection against mobile genetic elements (MGEs), yet a number of MGEs also harbor CRISPR-Cas components. With a few exceptions, CRISPR-Cas loci encoded on MGEs are uncharted and a comprehensive analysis of their distribution, prevalence, diversity, and function is lacking. Here, we systematically investigated CRISPR-Cas loci across the largest curated collection of natural bacterial and archaeal plasmids. CRISPR-Cas loci are widely but heterogeneously distributed across plasmids and, in comparison to host chromosomes, their mean prevalence per Mbp is higher and their distribution is distinct. Furthermore, the spacer content of plasmid CRISPRs exhibits a strong targeting bias towards other plasmids, while chromosomal arrays are enriched with virus-targeting spacers. These contrasting targeting preferences highlight the genetic independence of plasmids and suggest a major role for mediating plasmid-plasmid conflicts. Altogether, CRISPR-Cas are frequent accessory components of many plasmids, which is an overlooked phenomenon that possibly facilitates their dissemination across microbiomes.",
author = "Rafael Pinilla-Redondo and Jakob Russel and David Mayo-Mu{\~n}oz and Shah, {Shiraz A.} and Garrett, {Roger A.} and Joseph Nesme and Madsen, {Jonas S.} and Fineran, {Peter C.} and S{\o}rensen, {S{\o}ren J.}",
note = "{\textcopyright} The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2022",
doi = "10.1093/nar/gkab859",
language = "English",
volume = "50",
pages = "4315--4328",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "8",

}

RIS

TY - JOUR

T1 - CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids

AU - Pinilla-Redondo, Rafael

AU - Russel, Jakob

AU - Mayo-Muñoz, David

AU - Shah, Shiraz A.

AU - Garrett, Roger A.

AU - Nesme, Joseph

AU - Madsen, Jonas S.

AU - Fineran, Peter C.

AU - Sørensen, Søren J.

N1 - © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2022

Y1 - 2022

N2 - Many prokaryotes encode CRISPR-Cas systems as immune protection against mobile genetic elements (MGEs), yet a number of MGEs also harbor CRISPR-Cas components. With a few exceptions, CRISPR-Cas loci encoded on MGEs are uncharted and a comprehensive analysis of their distribution, prevalence, diversity, and function is lacking. Here, we systematically investigated CRISPR-Cas loci across the largest curated collection of natural bacterial and archaeal plasmids. CRISPR-Cas loci are widely but heterogeneously distributed across plasmids and, in comparison to host chromosomes, their mean prevalence per Mbp is higher and their distribution is distinct. Furthermore, the spacer content of plasmid CRISPRs exhibits a strong targeting bias towards other plasmids, while chromosomal arrays are enriched with virus-targeting spacers. These contrasting targeting preferences highlight the genetic independence of plasmids and suggest a major role for mediating plasmid-plasmid conflicts. Altogether, CRISPR-Cas are frequent accessory components of many plasmids, which is an overlooked phenomenon that possibly facilitates their dissemination across microbiomes.

AB - Many prokaryotes encode CRISPR-Cas systems as immune protection against mobile genetic elements (MGEs), yet a number of MGEs also harbor CRISPR-Cas components. With a few exceptions, CRISPR-Cas loci encoded on MGEs are uncharted and a comprehensive analysis of their distribution, prevalence, diversity, and function is lacking. Here, we systematically investigated CRISPR-Cas loci across the largest curated collection of natural bacterial and archaeal plasmids. CRISPR-Cas loci are widely but heterogeneously distributed across plasmids and, in comparison to host chromosomes, their mean prevalence per Mbp is higher and their distribution is distinct. Furthermore, the spacer content of plasmid CRISPRs exhibits a strong targeting bias towards other plasmids, while chromosomal arrays are enriched with virus-targeting spacers. These contrasting targeting preferences highlight the genetic independence of plasmids and suggest a major role for mediating plasmid-plasmid conflicts. Altogether, CRISPR-Cas are frequent accessory components of many plasmids, which is an overlooked phenomenon that possibly facilitates their dissemination across microbiomes.

U2 - 10.1093/nar/gkab859

DO - 10.1093/nar/gkab859

M3 - Journal article

C2 - 34606604

VL - 50

SP - 4315

EP - 4328

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 8

ER -

ID: 281224711