De novo assembly of human genomes with massively parallel short read sequencing

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

De novo assembly of human genomes with massively parallel short read sequencing. / Li, Ruiqiang; Zhu, Hongmei; Ruan, Jue; Qian, Wubin; Fang, Xiaodong; Shi, Zhongbin; Li, Yingrui; Li, Shengting; Shan, Gao; Kristiansen, Karsten; Li, Songgang; Yang, Huanming; Wang, Jian; Wang, Jun.

In: Genome Research, Vol. 20, No. 2, 2010, p. 265-72.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Li, R, Zhu, H, Ruan, J, Qian, W, Fang, X, Shi, Z, Li, Y, Li, S, Shan, G, Kristiansen, K, Li, S, Yang, H, Wang, J & Wang, J 2010, 'De novo assembly of human genomes with massively parallel short read sequencing', Genome Research, vol. 20, no. 2, pp. 265-72. https://doi.org/10.1101/gr.097261.109

APA

Li, R., Zhu, H., Ruan, J., Qian, W., Fang, X., Shi, Z., Li, Y., Li, S., Shan, G., Kristiansen, K., Li, S., Yang, H., Wang, J., & Wang, J. (2010). De novo assembly of human genomes with massively parallel short read sequencing. Genome Research, 20(2), 265-72. https://doi.org/10.1101/gr.097261.109

Vancouver

Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Research. 2010;20(2):265-72. https://doi.org/10.1101/gr.097261.109

Author

Li, Ruiqiang ; Zhu, Hongmei ; Ruan, Jue ; Qian, Wubin ; Fang, Xiaodong ; Shi, Zhongbin ; Li, Yingrui ; Li, Shengting ; Shan, Gao ; Kristiansen, Karsten ; Li, Songgang ; Yang, Huanming ; Wang, Jian ; Wang, Jun. / De novo assembly of human genomes with massively parallel short read sequencing. In: Genome Research. 2010 ; Vol. 20, No. 2. pp. 265-72.

Bibtex

@article{911cfbf0a62911df928f000ea68e967b,
title = "De novo assembly of human genomes with massively parallel short read sequencing",
abstract = "Next-generation massively parallel DNA sequencing technologies provide ultrahigh throughput at a substantially lower unit data cost; however, the data are very short read length sequences, making de novo assembly extremely challenging. Here, we describe a novel method for de novo assembly of large genomes from short read sequences. We successfully assembled both the Asian and African human genome sequences, achieving an N50 contig size of 7.4 and 5.9 kilobases (kb) and scaffold of 446.3 and 61.9 kb, respectively. The development of this de novo short read assembly method creates new opportunities for building reference sequences and carrying out accurate analyses of unexplored genomes in a cost-effective way.",
author = "Ruiqiang Li and Hongmei Zhu and Jue Ruan and Wubin Qian and Xiaodong Fang and Zhongbin Shi and Yingrui Li and Shengting Li and Gao Shan and Karsten Kristiansen and Songgang Li and Huanming Yang and Jian Wang and Jun Wang",
note = "Keywords: African Continental Ancestry Group; Asian Continental Ancestry Group; Genome, Human; Human Genome Project; Humans; Oligonucleotide Array Sequence Analysis; Sequence Alignment; Sequence Analysis, DNA",
year = "2010",
doi = "10.1101/gr.097261.109",
language = "English",
volume = "20",
pages = "265--72",
journal = "Genome Research",
issn = "1088-9051",
publisher = "Cold Spring Harbor Laboratory Press",
number = "2",

}

RIS

TY - JOUR

T1 - De novo assembly of human genomes with massively parallel short read sequencing

AU - Li, Ruiqiang

AU - Zhu, Hongmei

AU - Ruan, Jue

AU - Qian, Wubin

AU - Fang, Xiaodong

AU - Shi, Zhongbin

AU - Li, Yingrui

AU - Li, Shengting

AU - Shan, Gao

AU - Kristiansen, Karsten

AU - Li, Songgang

AU - Yang, Huanming

AU - Wang, Jian

AU - Wang, Jun

N1 - Keywords: African Continental Ancestry Group; Asian Continental Ancestry Group; Genome, Human; Human Genome Project; Humans; Oligonucleotide Array Sequence Analysis; Sequence Alignment; Sequence Analysis, DNA

PY - 2010

Y1 - 2010

N2 - Next-generation massively parallel DNA sequencing technologies provide ultrahigh throughput at a substantially lower unit data cost; however, the data are very short read length sequences, making de novo assembly extremely challenging. Here, we describe a novel method for de novo assembly of large genomes from short read sequences. We successfully assembled both the Asian and African human genome sequences, achieving an N50 contig size of 7.4 and 5.9 kilobases (kb) and scaffold of 446.3 and 61.9 kb, respectively. The development of this de novo short read assembly method creates new opportunities for building reference sequences and carrying out accurate analyses of unexplored genomes in a cost-effective way.

AB - Next-generation massively parallel DNA sequencing technologies provide ultrahigh throughput at a substantially lower unit data cost; however, the data are very short read length sequences, making de novo assembly extremely challenging. Here, we describe a novel method for de novo assembly of large genomes from short read sequences. We successfully assembled both the Asian and African human genome sequences, achieving an N50 contig size of 7.4 and 5.9 kilobases (kb) and scaffold of 446.3 and 61.9 kb, respectively. The development of this de novo short read assembly method creates new opportunities for building reference sequences and carrying out accurate analyses of unexplored genomes in a cost-effective way.

U2 - 10.1101/gr.097261.109

DO - 10.1101/gr.097261.109

M3 - Journal article

C2 - 20019144

VL - 20

SP - 265

EP - 272

JO - Genome Research

JF - Genome Research

SN - 1088-9051

IS - 2

ER -

ID: 21338168