Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation

Research output: Contribution to journalJournal articlepeer-review

Standard

Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation. / Bezy, Olivier; Elabd, Christian; Cochet, Olivia; Petersen, Rasmus Koefoed; Arkowitz, R.; Kristiansen, Karsten; fl., m.

In: Journal of Biological Chemistry, Vol. 280, No. 12, 2005, p. 11432-11438.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Bezy, O, Elabd, C, Cochet, O, Petersen, RK, Arkowitz, R, Kristiansen, K & fl., M 2005, 'Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation', Journal of Biological Chemistry, vol. 280, no. 12, pp. 11432-11438. https://doi.org/10.1074/jbc.M411741200

APA

Bezy, O., Elabd, C., Cochet, O., Petersen, R. K., Arkowitz, R., Kristiansen, K., & fl., M. (2005). Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation. Journal of Biological Chemistry, 280(12), 11432-11438. https://doi.org/10.1074/jbc.M411741200

Vancouver

Bezy O, Elabd C, Cochet O, Petersen RK, Arkowitz R, Kristiansen K et al. Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation. Journal of Biological Chemistry. 2005;280(12):11432-11438. https://doi.org/10.1074/jbc.M411741200

Author

Bezy, Olivier ; Elabd, Christian ; Cochet, Olivia ; Petersen, Rasmus Koefoed ; Arkowitz, R. ; Kristiansen, Karsten ; fl., m. / Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 12. pp. 11432-11438.

Bibtex

@article{09d0b810f75411ddbf70000ea68e967b,
title = "Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein {\ss}, Implicated in Adipocyte Differentiation",
abstract = "CCAAT/enhancer-binding protein {\ss} (C/EBP{\ss}) is expressed early during the adipocyte differentiation program and plays an important role in this process. In an attempt to identify novel proteins that interact with C/EBP{\ss}, we performed a yeast two-hybrid screen with a preadipocyte cDNA library and identified a new co-regulator, delta-interacting protein A (DIPA). DIPA mRNA is expressed during adipocyte differentiation of clonal cell lines. DIPA interacts with C/EBP{\ss} and -d proteins in intact cells and inhibits their transcriptional activity but not that of C/EBPa. Stable overexpression of DIPA in preadipocytes partially inhibits adipocyte differentiation, whereas its gene silencing enhances this process. DIPA and C/EBP{\ss} co-localize in the nucleus, and overexpression of DIPA in preadipocytes results in a partial inhibition of the mitotic clonal expansion which is critical for differentiation. Thus, DIPA is a novel partner of C/EBP{\ss} that down-regulates early events of adipogenesis. ",
author = "Olivier Bezy and Christian Elabd and Olivia Cochet and Petersen, {Rasmus Koefoed} and R. Arkowitz and Karsten Kristiansen and m. fl.",
year = "2005",
doi = "10.1074/jbc.M411741200",
language = "English",
volume = "280",
pages = "11432--11438",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "12",

}

RIS

TY - JOUR

T1 - Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation

AU - Bezy, Olivier

AU - Elabd, Christian

AU - Cochet, Olivia

AU - Petersen, Rasmus Koefoed

AU - Arkowitz, R.

AU - Kristiansen, Karsten

AU - fl., m.

PY - 2005

Y1 - 2005

N2 - CCAAT/enhancer-binding protein ß (C/EBPß) is expressed early during the adipocyte differentiation program and plays an important role in this process. In an attempt to identify novel proteins that interact with C/EBPß, we performed a yeast two-hybrid screen with a preadipocyte cDNA library and identified a new co-regulator, delta-interacting protein A (DIPA). DIPA mRNA is expressed during adipocyte differentiation of clonal cell lines. DIPA interacts with C/EBPß and -d proteins in intact cells and inhibits their transcriptional activity but not that of C/EBPa. Stable overexpression of DIPA in preadipocytes partially inhibits adipocyte differentiation, whereas its gene silencing enhances this process. DIPA and C/EBPß co-localize in the nucleus, and overexpression of DIPA in preadipocytes results in a partial inhibition of the mitotic clonal expansion which is critical for differentiation. Thus, DIPA is a novel partner of C/EBPß that down-regulates early events of adipogenesis.

AB - CCAAT/enhancer-binding protein ß (C/EBPß) is expressed early during the adipocyte differentiation program and plays an important role in this process. In an attempt to identify novel proteins that interact with C/EBPß, we performed a yeast two-hybrid screen with a preadipocyte cDNA library and identified a new co-regulator, delta-interacting protein A (DIPA). DIPA mRNA is expressed during adipocyte differentiation of clonal cell lines. DIPA interacts with C/EBPß and -d proteins in intact cells and inhibits their transcriptional activity but not that of C/EBPa. Stable overexpression of DIPA in preadipocytes partially inhibits adipocyte differentiation, whereas its gene silencing enhances this process. DIPA and C/EBPß co-localize in the nucleus, and overexpression of DIPA in preadipocytes results in a partial inhibition of the mitotic clonal expansion which is critical for differentiation. Thus, DIPA is a novel partner of C/EBPß that down-regulates early events of adipogenesis.

U2 - 10.1074/jbc.M411741200

DO - 10.1074/jbc.M411741200

M3 - Journal article

C2 - 15644333

VL - 280

SP - 11432

EP - 11438

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 12

ER -

ID: 10242468