Design and setup of intermittent-flow respirometry system for aquatic organisms

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Design and setup of intermittent-flow respirometry system for aquatic organisms. / Svendsen, Morten Bo Søndergaard; Bushnell, P.G.; Steffensen, John Fleng.

In: Journal of Fish Biology, Vol. 88, No. 1, 2016, p. 26-50.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Svendsen, MBS, Bushnell, PG & Steffensen, JF 2016, 'Design and setup of intermittent-flow respirometry system for aquatic organisms', Journal of Fish Biology, vol. 88, no. 1, pp. 26-50. https://doi.org/10.1111/jfb.12797

APA

Svendsen, M. B. S., Bushnell, P. G., & Steffensen, J. F. (2016). Design and setup of intermittent-flow respirometry system for aquatic organisms. Journal of Fish Biology, 88(1), 26-50. https://doi.org/10.1111/jfb.12797

Vancouver

Svendsen MBS, Bushnell PG, Steffensen JF. Design and setup of intermittent-flow respirometry system for aquatic organisms. Journal of Fish Biology. 2016;88(1):26-50. https://doi.org/10.1111/jfb.12797

Author

Svendsen, Morten Bo Søndergaard ; Bushnell, P.G. ; Steffensen, John Fleng. / Design and setup of intermittent-flow respirometry system for aquatic organisms. In: Journal of Fish Biology. 2016 ; Vol. 88, No. 1. pp. 26-50.

Bibtex

@article{cb83cd88c5a4469a9b69ab9f9a2ca79d,
title = "Design and setup of intermittent-flow respirometry system for aquatic organisms",
abstract = "Intermittent-flow respirometry is an experimental protocol for measuring oxygen consumption in aquatic organisms that utilizes the best features of closed (stop-flow) and flow-through respirometry while eliminating (or at least reducing) some of their inherent problems. By interspersing short periods of closed-chamber oxygen consumption measurements with regular flush periods, accurate oxygen uptake rate measurements can be made without the accumulation of waste products, particularly carbon dioxide, which may confound results. Automating the procedure with easily available hardware and software further reduces error by allowing many measurements to be made over long periods thereby minimizing animal stress due to acclimation issues. This paper describes some of the fundamental principles that need to be considered when designing and carrying out automated intermittent-flow respirometry (e.g. chamber size, flush rate, flush time, chamber mixing, measurement periods and temperature control). Finally, recent advances in oxygen probe technology and open source automation software will be discussed in the context of assembling relatively low cost and reliable measurement systems.",
author = "Svendsen, {Morten Bo S{\o}ndergaard} and P.G. Bushnell and Steffensen, {John Fleng}",
note = "{\textcopyright} 2015 The Fisheries Society of the British Isles.",
year = "2016",
doi = "10.1111/jfb.12797",
language = "English",
volume = "88",
pages = "26--50",
journal = "Journal of Fish Biology",
issn = "0022-1112",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Design and setup of intermittent-flow respirometry system for aquatic organisms

AU - Svendsen, Morten Bo Søndergaard

AU - Bushnell, P.G.

AU - Steffensen, John Fleng

N1 - © 2015 The Fisheries Society of the British Isles.

PY - 2016

Y1 - 2016

N2 - Intermittent-flow respirometry is an experimental protocol for measuring oxygen consumption in aquatic organisms that utilizes the best features of closed (stop-flow) and flow-through respirometry while eliminating (or at least reducing) some of their inherent problems. By interspersing short periods of closed-chamber oxygen consumption measurements with regular flush periods, accurate oxygen uptake rate measurements can be made without the accumulation of waste products, particularly carbon dioxide, which may confound results. Automating the procedure with easily available hardware and software further reduces error by allowing many measurements to be made over long periods thereby minimizing animal stress due to acclimation issues. This paper describes some of the fundamental principles that need to be considered when designing and carrying out automated intermittent-flow respirometry (e.g. chamber size, flush rate, flush time, chamber mixing, measurement periods and temperature control). Finally, recent advances in oxygen probe technology and open source automation software will be discussed in the context of assembling relatively low cost and reliable measurement systems.

AB - Intermittent-flow respirometry is an experimental protocol for measuring oxygen consumption in aquatic organisms that utilizes the best features of closed (stop-flow) and flow-through respirometry while eliminating (or at least reducing) some of their inherent problems. By interspersing short periods of closed-chamber oxygen consumption measurements with regular flush periods, accurate oxygen uptake rate measurements can be made without the accumulation of waste products, particularly carbon dioxide, which may confound results. Automating the procedure with easily available hardware and software further reduces error by allowing many measurements to be made over long periods thereby minimizing animal stress due to acclimation issues. This paper describes some of the fundamental principles that need to be considered when designing and carrying out automated intermittent-flow respirometry (e.g. chamber size, flush rate, flush time, chamber mixing, measurement periods and temperature control). Finally, recent advances in oxygen probe technology and open source automation software will be discussed in the context of assembling relatively low cost and reliable measurement systems.

U2 - 10.1111/jfb.12797

DO - 10.1111/jfb.12797

M3 - Journal article

C2 - 26603018

VL - 88

SP - 26

EP - 50

JO - Journal of Fish Biology

JF - Journal of Fish Biology

SN - 0022-1112

IS - 1

ER -

ID: 149044366