Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast. / Mandrup, S; Jepsen, R; Skøtt, H; Rosendal, J; Højrup, P; Kristiansen, K; Knudsen, J.

In: Biochemical Journal, Vol. 290 ( Pt 2), 1993, p. 369-74.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mandrup, S, Jepsen, R, Skøtt, H, Rosendal, J, Højrup, P, Kristiansen, K & Knudsen, J 1993, 'Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast', Biochemical Journal, vol. 290 ( Pt 2), pp. 369-74. <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1132282>

APA

Mandrup, S., Jepsen, R., Skøtt, H., Rosendal, J., Højrup, P., Kristiansen, K., & Knudsen, J. (1993). Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast. Biochemical Journal, 290 ( Pt 2), 369-74. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1132282

Vancouver

Mandrup S, Jepsen R, Skøtt H, Rosendal J, Højrup P, Kristiansen K et al. Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast. Biochemical Journal. 1993;290 ( Pt 2):369-74.

Author

Mandrup, S ; Jepsen, R ; Skøtt, H ; Rosendal, J ; Højrup, P ; Kristiansen, K ; Knudsen, J. / Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast. In: Biochemical Journal. 1993 ; Vol. 290 ( Pt 2). pp. 369-74.

Bibtex

@article{0cf1e9c00f0411de8478000ea68e967b,
title = "Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast",
abstract = "We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.",
author = "S Mandrup and R Jepsen and H Sk{\o}tt and J Rosendal and P H{\o}jrup and K Kristiansen and J Knudsen",
note = "Keywords: Acyl Coenzyme A; Animals; Base Sequence; Carrier Proteins; Cattle; Cloning, Molecular; Escherichia coli; Fatty Acid-Binding Proteins; Molecular Sequence Data; Neoplasm Proteins; Oligonucleotides; Plasmids; Promoter Regions, Genetic; Recombinant Proteins; Saccharomyces cerevisiae",
year = "1993",
language = "English",
volume = "290 ( Pt 2)",
pages = "369--74",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",

}

RIS

TY - JOUR

T1 - Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast

AU - Mandrup, S

AU - Jepsen, R

AU - Skøtt, H

AU - Rosendal, J

AU - Højrup, P

AU - Kristiansen, K

AU - Knudsen, J

N1 - Keywords: Acyl Coenzyme A; Animals; Base Sequence; Carrier Proteins; Cattle; Cloning, Molecular; Escherichia coli; Fatty Acid-Binding Proteins; Molecular Sequence Data; Neoplasm Proteins; Oligonucleotides; Plasmids; Promoter Regions, Genetic; Recombinant Proteins; Saccharomyces cerevisiae

PY - 1993

Y1 - 1993

N2 - We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.

AB - We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.

M3 - Journal article

C2 - 8452523

VL - 290 ( Pt 2)

SP - 369

EP - 374

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

ER -

ID: 11231151